...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biochemical and molecular toxicology >Downregulating STAT1/caspase‐3 signaling with fludarabine to alleviate progression in a rat model of steroid‐induced avascular necrosis of the femoral head
【24h】

Downregulating STAT1/caspase‐3 signaling with fludarabine to alleviate progression in a rat model of steroid‐induced avascular necrosis of the femoral head

机译：下调STAT1 / caspase-3与氟咔啉的信号传导，缓解类固醇诱导的股骨头缺血性坏死大鼠模型的进展

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract Steroid‐induced avascular necrosis of the femoral head (SANFH) is mainly induced by glucocorticoids. Fludarabine (Flu) is a specific signal transducer and activator of transcription 1 (STAT1) inhibitor. In this study, we investigated the effect of Flu on SANFH and the role played by the STAT1/caspase‐3 signaling pathway. Sprague‐Dawley rats were divided into control, SANFH, and Flu‐treated SANFH groups. Femoral head tissues were collected for hematoxylin‐eosin (H&E) staining and Western blot analysis. The latter was used to measure the levels of stat1, phospho‐stat1, caspase‐3, cleaved caspase‐3, caspase‐9, cleaved caspase‐9, Bax, cytochrome C, Bak, B‐cell lymphoma‐extra large, and?B‐cell lymphoma‐2 protein expression. The results showed that Flu regulates protein expression in dexamethasone (Dex)‐induced SANFH. H&E staining showed a decrease in the ratio of empty lacunae induced by Dex. Taken together, our study demonstrated the involvement of the STAT1/caspase‐3 signaling pathway in SANFH and the potential of Flu as a therapeutic agent for patients with SANFH.
机译：摘要股骨头（SANFH）的类固醇诱导的缺血性坏死主要由糖皮质激素诱导。 Fludarabine（流感）是一种特定的信号传感器和转录1（Stat1）抑制剂的活化剂。在这项研究中，我们研究了流感对Sanfh的影响，STAT1 / Caspase-3信号通路的作用。 Sprague-Dawley大鼠分为对照，Sanfh和流感治疗的Sanfh组。收集股骨头组织，用于苏木精 - 曙红（H＆amp; e）染色和Western印迹分析。后者用于测量Stat1，磷酸-TAT1，caspase-3，切割的caspase-3，caspase-9，裂解的caspase-9，Bax，细胞色素C，Bak，B细胞淋巴瘤 - 超大，以及？ B细胞淋巴瘤-2蛋白表达。结果表明，流感调节地塞米松（DEX）诱导的SanFH中的蛋白质表达。 H＆amp; E染色显示DEX诱导的空裂变术的比例降低。我们的研究占据了，我的研究表明，SATFH中的STAT1 / caspase-3信号传导途径和流感潜力作为Sanfh患者的治疗剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号