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首页> 外文期刊>Journal of biochemical and molecular toxicology >Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo in vivo and on erythrocyte G6PD in vitro in vitro in rats
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Effect of astaxanthin and aluminum chloride on erythrocyte G6PD and 6PGD enzyme activities in vivo in vivo and on erythrocyte G6PD in vitro in vitro in rats

机译：虾青素和氯化铝对大鼠体外体内和红细胞G6PD体内红细胞G6PD和6PGD酶活性的影响

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摘要

Abstract In this study, we investigated the effect of astaxanthin (Ast) and aluminum (Al) on the erythrocyte glucose‐6‐phosphate dehydrogenase (G6PD) and 6‐phosphogluconate dehydrogenase (6PGD) enzymes activities in vivo and on G6PD enzyme in vitro in rats. For in vitro studies, G6PD enzyme was purified from rat erythrocyte by using 2′,5′‐ADP‐Sepharose 4B affinity gel. The effects of Ast and Al 3+ ion were investigated on the purified enzyme. It was determined that Ast increased the enzyme activity, whereas Al 3+ inhibited the enzyme activity noncompetitively (IC 50 values; 0.679?mM, K i values 1.32?mM). For in vivo studies, the rats were divided into the groups: control (Cont.), Al, Ast, and Al?+?Ast. The last three groups were compared with the control group. In Al group, a significant degree of inhibition was observed in the activity of G6PD and 6PGD enzymes when compared with the control group ( P ??0.05), whereas there was an increase in the activities of G6PD and 6PGD enzymes in Ast and Al?+?Ast groups ( P ??0.05).
机译：摘要在这项研究中，我们研究了虾青素（AST）和铝（AL）对体内和体外G6PD酶的红细胞葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷光葡糖苷脱氢酶（6PGD）酶活性的影响老鼠。对于体外研究，通过使用2'，5'-ADP-Sepharose 4B亲和性凝胶从大鼠红细胞纯化G6PD酶。在纯化的酶上研究了AST和Al 3+离子的效果。确定AST增加了酶活性，而Al 3+抑制了非竞争力的酶活性（IC 50值;0.679Ωmm，k i值1.32Ωmm）。对于体内研究，将大鼠分为组：对照（续），Al，AST和Al？+αAST。最后三组与对照组进行比较。在Al组中，在与对照组（p≤0.05）相比时在G6PD和6PGD酶的活性中观察到显着程度的抑制程度，而AST和6PGD酶的活性增加Al？+αstrα（p≤≤0.05）。

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