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首页> 外文期刊>Journal of biochemical and molecular toxicology >Regulation of the GST Mu-1 isoenzyme in Y1 cells by adrenocorticotropic hormone is primarily transcriptional.
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Regulation of the GST Mu-1 isoenzyme in Y1 cells by adrenocorticotropic hormone is primarily transcriptional.

机译：通过肾上腺激素激素调节Y1细胞中的GST mu-1同工酶主要是转录的。

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摘要

In earlier experiments, adrenocorticotropic hormone (ACTH) was shown to decrease the level of glutathione transferase M1 (murine glutathione transferase mu-1) (mGSTM1), as well as of the corresponding mRNA, in a murine adrenocortical cell line. In the present study, the effect of ACTH on mGSTM1 gene transcription was examined using two techniques. First, a cDNA that coded for the mGSTM1 subunit but lacked the corresponding promoter sequences was transfected into the adrenocortical cell line, and the effect of ACTH on the level of the corresponding transcript was compared to that of endogenous mGSTM1 mRNA. The other technique used was nuclear run-on transcription, where the rate of transcription of endogenous mGSTM1 mRNA in ACTH-treated cells was compared to that in untreated control cells. These experimental approaches indicated that the rate of transcription of the mGSTM1 gene is regulated by ACTH in adrenocortical cells. Copyright 2001 John Wiley & Sons, Inc. J Biochem Mol Toxicol 15:128-132, 2001
机译：在早期的实验中，显示肾上腺皮质激素（ACTH），降低谷胱甘肽转移酶M1（鼠谷胱甘肽转移酶MU-1）（MGSTM1）的水平，以及相应的mRNA，在鼠肾上腺皮质细胞系中。在本研究中，使用两种技术检查ACTH对MgSTM1基因转录的影响。首先，将编码用于MgStM1亚基但缺乏相应的启动子序列的cDNA转染到肾上腺皮质细胞系中，并将ACTH对相应转录物水平的影响与内源Mgstm1 mRNA进行比较。使用的其他技术是核循环转录，其中将内源性Mgstm1 mRNA在非处理的对照细胞中进行了内源性Mgstm1 mRNA的转录速率。这些实验方法表明，MgSTM1基因的转录速率由肾上腺皮质细胞中的acth调节。版权所有2001 John Wiley＆Sons，Inc .J Biochem Mol毒剂15：128-132,2001

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