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首页> 外文期刊>Journal of biochemical and molecular toxicology >Metabolism of phenanthrene by house fly CYP6D1 and dog liver cytochrome P450.
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Metabolism of phenanthrene by house fly CYP6D1 and dog liver cytochrome P450.

机译：房屋飞CYP6D1和狗肝细胞色素P450的菲代谢的菲代谢。

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摘要

Polycyclic aromatic hydrocarbons (PAHs) are a ubiquitous class of environmental contaminants. The compound phenanthrene is a model PAH. A novel fluorometric method for measuring phenanthrene metabolism in vitro was developed and verified with direct measurement of [14C]phenanthrene using dog liver microsomes. The fluorometric assay and direct measurement of [14C]phenanthrene metabolism were used to show that CYP6D1, a house fly cytochrome P450, is the major house fly P450 involved in phenanthrene metabolism. Phenanthrene was metabolized by microsomes from the LPR strain of house fly that overexpresses CYP6D1, but metabolism was not observed in the CS strain that has a lower level of CYP6D1. Furthermore, the majority of phenanthrene metabolism was inhibited by a CYP6D1-specific antibody. This study increases the number of known substrates of CYP6D1 and identifies polyaromatic hydrocarbons as potential substrates of CYP6D1. The utility of CYP6D1 as an agent in bioremediation and the utility of the new fluorometric assay for understanding PAH metabolism in insects and mammals are discussed.
机译：多环芳烃（PAH）是一类普遍存在的环境污染物。复合菲是一种模型PAH。开发并验证了一种新的荧光法，用于使用狗肝微粒体直接测量[14C]菲的[14C]菲的直接测量。荧光测定和[14C]菲代谢的直接测量用于表明CYP6D1，房屋飞行细胞色素P450，是菲苯甲烷代谢的主要房屋飞行P450。通过从LPR的LPR菌株的微粒体来代谢苯丙烯，从而过度表达CYP6D1，但在具有较低水平的CYP6D1的CS菌株中未观察到代谢。此外，大多数菲代谢由CYP6D1特异性抗体抑制。该研究增加了CYP6D1的已知底物的数量，并将多芳烃鉴定为CYP6D1的潜在基板。讨论了CYP6D1作为生物修复药物的效用以及用于了解昆虫和哺乳动物中PAH代谢的新荧光测定的效用。

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