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首页> 外文期刊>Journal of biomedical materials research, Part A >Study on in vitro release and cell response to alendronate sodium-loaded ultrahigh molecular weight polyethylene loaded with alendronate sodium wear particles to treat the particles-induced osteolysis.
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Study on in vitro release and cell response to alendronate sodium-loaded ultrahigh molecular weight polyethylene loaded with alendronate sodium wear particles to treat the particles-induced osteolysis.

机译：含有Alynate钠磨损颗粒的Alendronate钠加载超高分子量聚乙烯的体外释放和细胞反应的研究。

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摘要

The aim of this study is to investigate in vitro release and cell response to wear particles of ultrahigh molecular weight polyethylene loaded with alendronate sodium (UHMWPE-ALN), a potent bone resorption inhibitor. Wear particles of UHMWPE-ALN with different ALN contents (0.5 wt % or 1.0 wt %) and size ranges (<45 μm or 45-75 μm) were cocultured with macrophages (RAW264.7) and osteoblasts (MC3T3-E1), respectively. The in vitro ALN release was divided into three stages: an initial burst release, subsequent rapid release, and final slow release. The particle size and ALN content of UHMWPE-ALN wear particles affected the in vitro release mainly during initial burst and rapid release. Compared with the control cells, UHMWPE-ALN wear particles stimulated a significant elevation of tumor necrosis factor-alpha (TNF-α) release from macrophages but had no obvious effect on interleukin-6 release. However, this stimulation of TNF-α release could be reduced by ALN released from UHMWPE-ALN wear particles. The wear particle size had stronger effect of on the macrophages compared with the ALN concentration. After coculture with UHMWPE-ALN wear particles, osteoblast proliferation and alkaline phosphatase activities increased moderately with the increase in particle sizes and ALN concentrations. These results suggest that incorporation of ALN in UHMWPE-ALN may be an effective approach to prevent or reduce particles-induced osteolysis.
机译：本研究的目的是研究含有常规分子量聚乙烯的磨损颗粒的体外释放和细胞响应，其中有效的骨吸收抑制剂。用不同的AlN含量（0.5wt％或1.0wt％）和尺寸范围（<45μm或45-75μm）分别与巨噬细胞（Raw264.7）和成骨细胞（MC3T3-E1）进行磨损UHMWPE-ALN颗粒。将体外AlN释放分为三个阶段：初始爆发释放，随后的快速释放和最终缓慢释放。 UHMWPE-ALN磨损颗粒的粒度和ALN含量主要在初始突发和快速释放期间影响体外释放。与对照细胞相比，UHMWPE-ALN磨损颗粒刺激肿瘤坏死因子-α（TNF-α）释放的显着升高，但对白细胞介素-6释放没有明显的影响。然而，通过从UHMWPE-ALN磨损颗粒释放的ALN可以减少这种TNF-α释放的这种刺激。与ALN浓度相比，磨损粒度对巨噬细胞具有更强的效果。通过UHMWPE-ALN磨损颗粒与UHMWPE-ALN磨损颗粒，骨细胞增殖和碱性磷酸酶活性较高，随着颗粒尺寸和ALN浓度的增加而增加。这些结果表明，在UHMWPE-ALN中掺入ALN可能是预防或减少颗粒诱导的骨解的有效方法。

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