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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >The effect of collector type on the physical, chemical, and biological properties of polycaprolactone/gelatin/nano-hydroxyapatite electrospun scaffold
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The effect of collector type on the physical, chemical, and biological properties of polycaprolactone/gelatin/nano-hydroxyapatite electrospun scaffold

机译：收集器型对聚己内酯/明胶/纳米羟基磷灰石电纺支架的物理，化学和生物学性质的影响

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摘要

Electrospinning is considered a powerful method for the production of fibers in the nanoscale size. Small pore size results in poor cell infiltration, cell migration inhibition into scaffold pores and low oxygen diffusion. Electrospun polycaprolactone/gelatin/nano-hydroxyapatite (PCL/Gel/nHA) scaffolds were deposited into two types of fiber collectors (novel rotating disc and plate) to study fiber morphology, chemical, mechanical, hydrophilic, and biodegradation properties between each other. The proliferation and differentiation of MG-63 cells into the bone phenotype were determined using MTT method, alizarin red staining and alkaline phosphatase (ALP) activity. The rates for disc rotation were 50 and 100 rpm. The pore size measurement results indicated that the fibers produced by the disc rotation collector with speed rate 50 rpm have larger pores as compared to fibers produced by disc rotation at 100 rpm and flat plate collectors. A randomly structure with controlled pore size (38.65 +/- 0.33 mu m) and lower fiber density, as compared to fibers collected by disc rotation with speed rate 100 rpm and flat plate collectors, was obtained. Fibers collected on the rotating disc with speed rate 50 rpm, were more hydrophilic due to larger pore size and therefore, faster infiltration of water into the scaffold and the rate of degradation was higher. These results demonstrate that PCL/Gel/nHA scaffolds made through a rotating disc collector at 50 rpm are more feasible to be used in bone tissue engineering applications due to appropriate pore size and increased adhesion and proliferation of cells, ALP activity and mineral deposits. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 933-950, 2019.
机译：静电纺丝被认为是纳米级尺寸中纤维的强大方法。小孔尺寸导致细胞浸润不良，细胞迁移抑制成支架孔和低氧扩散。将电纺聚碳酮/明胶/纳米 - 羟基磷灰石（PCL / GEL / NHA）支架沉积成两种类型的纤维收集器（新型旋转盘和板），以研究彼此之间的纤维形态，化学，机械，亲水和生物降解性能。使用MTT方法，茜素红染色和碱性磷酸酶（ALP）活性测定Mg-63细胞的增殖和分化。盘旋转的速率为50和100 rpm。孔径测量结果表明，与通过在100rpm和平板收集器产生的盘旋转产生的纤维相比，由圆盘旋转收集器产生的纤维具有较大的孔。得到了受控孔径（38.65 +/-0.33μm）和较低纤维密度的随机结构，与通过速度率100rpm和平板收集器收集的纤维相比。由于较大的孔径为速度50rpm收集在旋转盘上的纤维，因此孔径较大，因此，水进入支架的速度更快，降解速率较高。这些结果表明，由于适当的孔径和细胞，ALP活性和矿物沉积物增加，通过旋转盘收集器制备的PCL /凝胶/ NHA支架在50rpm下以50rpm制造的旋转盘收集器更加可行。 （c）2018 Wiley期刊，Inc.J生物保解员B部分B：苹果生物粉末107B：933-950,2019。

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