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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >The establishment and biological assessment of a whole tissue-engineered intervertebral disc with PBST fibers and a chitosan hydrogel in vitro and in vivo
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The establishment and biological assessment of a whole tissue-engineered intervertebral disc with PBST fibers and a chitosan hydrogel in vitro and in vivo

机译：整个组织工程椎间盘与PBST纤维和壳聚糖水凝胶的建立和生物学评估在体外和体内壳聚糖水凝胶

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Intervertebral disc (IVD) degeneration (IDD) is the main cause of low back pain in the clinic. In the advanced stage of IDD, both cell transplantation and gene therapy have obvious limitations. At this stage, tissue-engineered IVDs (TE-IVDs) provide new hope for the treatment of this disease. We aimed to construct a TE-IVD with a relatively complete structure. The inner annulus fibrosus (AF) was constructed using poly (butylene succinate-co-terephthalate) copolyester (PBST) electrospun fibers, and the outer AF consisted of solid PBST. The nucleus pulposus (NP) scaffold was constructed using a chitosan hydrogel, as reported in our previous research. The three components were assembled in vitro, and the mechanical properties were analyzed. AF and NP cells were implanted on the corresponding scaffolds. Then, the cell-seeded scaffolds were implanted subcutaneously in nude mice and cultured for 4 weeks; then they were removed and implanted into New Zealand white rabbits. After 4 weeks, their properties were analyzed. The PBST outer AF provided mechanical support for the whole TE-IVD. The electrospun film and chitosan hydrogel simulated the natural structure of the IVD well. Its mechanical property could meet the requirement of the normal IVD. Four weeks later, X-ray and MR imaging examination results suggested that the height of the intervertebral space was retained. The cells on the TE-IVD expressed extracellular matrix, which indicated that the cells maintained their biological function. Therefore, we conclude that the whole TE-IVD has biological and biomechanical properties to some extent, which is a promising candidate for IVD replacement therapies. (c) 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 2305-2316, 2019.
机译：椎间盘（IVD）变性（IDD）是临床腰部腰痛的主要原因。在IDD的晚期阶段，两种细胞移植和基因治疗都具有明显的局限性。在这个阶段，组织工程化IVDS（TE-IVDS）为治疗这种疾病提供了新的希望。我们旨在构建具有相对完整的结构的TE-IVD。内环纤维（AF）是使用聚（丁二烯酸丁二醇酯 - 对苯二甲酸酯）共聚酯（PBST）电纺丝纤维构建的，外部AF由固体PBST组成。如我们以前的研究中所报道的，使用壳聚糖水凝胶构建核瓜膜（NP）支架。在体外组装三种组分，分析机械性能。将AF和NP细胞植入相应的支架上。然后，将细胞播种的支架皮下植入裸鼠中并培养4周;然后他们被移除并植入新西兰白兔。 4周后，分析了它们的性质。 PBST外部AF为整个TE-IVD提供机械支撑。电纺膜和壳聚糖水凝胶模拟IVD井的自然结构。它的机械性能可以满足正常IVD的要求。四周后，X射线和MR成像检查结果表明椎间空间的高度被保留。 TE-IVD表达细胞外基质上的细胞，表明细胞保持其生物学功能。因此，我们得出结论，整个TE-IVD在一定程度上具有生物和生物力学性质，这是IVD替代疗法的有希望的候选者。 （c）2019 Wiley期刊，Inc。J生物保解率B：Appl Biomater 107B：2305-2316，2019。

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