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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Cotton wool-like nanocomposite biomaterials prepared by electrospinning: in vitro bioactivity and osteogenic differentiation of human mesenchymal stem cells.
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Cotton wool-like nanocomposite biomaterials prepared by electrospinning: in vitro bioactivity and osteogenic differentiation of human mesenchymal stem cells.

机译：通过静电纺丝制备棉羊毛状纳米复合生物材料：体外生物活性和人间充质干细胞的成骨分化。

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摘要

The present study evaluates the in vitro biomedical performance of an electrospun, flexible, and cotton wool-like poly(lactide-co-glycolide) (PLGA)/amorphous tricalcium phosphate (ATCP) nanocomposite. Experiments on in vitro biomineralization, applicability in model defects and a cell culture study with human mesenchymal stem cells (hMSC) allowed assessing the application of the material for potential use as a bone graft. Scaffolds with different flame made ATCP nanoparticle loadings were prepared by electrospinning of a PLGA-based composite. Immersion in simulated body fluid showed significant deposition of a hydroxyapatite layer only on the surface of ATCP doped PLGA (up to 175% mass gain within 15 days for PLGA/ATCP 60:40). Proliferation and osteogenic differentiation of hMSC on different nanocomposites were assessed by incubating cells in osteogenic medium for 4 weeks. Proper adhesion and an unaffected morphology of the cells were observed by confocal laser scanning microscopy for all samples. Fluorometric quantification of dsDNA and analysis of ALP activity revealed no significant difference between the tested scaffolds and excluded any acute cytotoxic effects of the nanoparticles. The osteocalcin content for all scaffolds was 0.12-0.19 ng/ng DNA confirming osteogenic differentiation of human mesenchymal stem cells on these flexible bone implants.
机译：本研究评估了电纺，柔性和棉羊毛状聚（丙交酯 - 共乙酰基）（PLGA）/非晶三钙（ATCP）纳米复合材料的体外生物医学性能。对体外生物碳化的实验，模型缺陷的适用性和人间充质干细胞（HMSC）的细胞培养研究允许评估材料施加作为骨移植物的潜在用途。采用不同火焰的支架制备ATCP纳米颗粒载体通过静电纺丝制备基于PLGA的复合材料。浸入模拟体液中仅在ATCP掺杂PLGA的表面上显着沉积羟基磷灰石层（在15天内高达175％质量增加，PLGA / ATCP 60:40）。通过在成骨培养基中孵育4周，评估HMSC在不同纳米复合材料上的增殖和骨质发生分化。通过共聚焦激光扫描显微镜对所有样品进行适当的粘附和细胞的未受影响的形态。 DSDNA的荧光定量和ALP活性的分析显示，测试的支架之间没有显着差异，并排除了纳米颗粒的任何急性细胞毒性作用。所有支架的骨钙素含量为0.12-0.19 ng / ng DNA，证明人间充质干细胞对这些柔性骨植入物的骨质发生分化。

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