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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >The application of a thermoresponsive chitosan/beta-GP gel to enhance cell repopulation of decellularized vascular scaffolds
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The application of a thermoresponsive chitosan/beta-GP gel to enhance cell repopulation of decellularized vascular scaffolds

机译：热响应壳聚糖/β-GP凝胶的应用增强脱细胞血管支架的细胞呕吐

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摘要

Decellularized arterial scaffolds have achieved success in advancing towards clinical use for small diameter vascular graft applications. Issues remain with effectively cell seeding these scaffolds, which may result in slow remodeling in vivo and reduced patency rates. This study aims to efficiently bulk load decellularized arterial scaffolds with cells and/or growth factors to promote cell migration within the scaffold. A chitosan/beta-glycerophosphate (beta-GP) hydrogel was used as a delivery vehicle for scaffold repopulation with rat mesenchymal stem cells (rMSCs) and/or murine hepatocyte growth factor (HGF). The thermoresponsivity of the chitosan/beta-GP HGF gel was determined by rheological analysis and the release of HGF by ELISA. The chitosan/beta-GP gel was fully injectable with gelation at 30 degrees C in similar to 30 min and demonstrated sustained controlled HGF release up to 28 days. Encapsulation of rMSCs allowed for consistent efficient cell delivery within the scaffold. Directional migration toward the released HGF was demonstrated within the medial layer of the scaffold up to a distance of 3 mm. The gel/cell/growth factor combination demonstrates a means of accelerating scaffold repopulation, without costly excessive in vitro maturation times. (C) 2014 Wiley Periodicals, Inc.
机译：脱细胞的动脉支架已经取得了成功的推进探索小直径血管移植应用的临床用途。有效地饲养这些支架的问题，这可能导致体内缓慢重塑和降低的通用速率。本研究旨在有效地批量负荷致泪动脉支架，其具有细胞和/或生长因子，以促进支架内的细胞迁移。用大鼠间充质干细胞（RMSCs）和/或鼠肝细胞生长因子（HGF）用作壳聚糖/β-甘油磷酸盐（Beta-GP）水凝胶作为支架重新灌注的递送载体。通过ELISA通过流变分析和HGF释放来确定壳聚糖/β-GP HGF凝胶的热反应性。壳聚糖/β-GP凝胶在30摄氏度下完全注射到30摄氏度，类似于30分钟，并且持续控制的HGF释放至28天。封装RMSCs允许在支架内允许一致的有效细胞输送。向释放的HGF的方向迁移在支架的内侧层内达到3mm的距离。凝胶/细胞/生长因子组合表明了一种加速支架重新灌注的方法，而没有昂贵的体外成熟时间。 （c）2014 Wiley期刊，Inc。

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