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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Behavior of bone cells in contact with magnesium implant material
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Behavior of bone cells in contact with magnesium implant material

机译：骨细胞与镁植入材料接触的行为

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Magnesium-based implants exhibit several advantages, such as biodegradability and possible osteoinductive properties. Whether the degradation may induce cell type-specific changes in metabolism still remains unclear. To examine the osteoinductivity mechanisms, the reaction of bone-derived cells (MG63, U2OS, SaoS2, and primary human osteoblasts (OB)) to magnesium (Mg) was determined. Mg-based extracts were used to mimic more realistic Mg degradation conditions. Moreover, the influence of cells having direct contact with the degrading Mg metal was investigated. In exposure to extracts and in direct contact, the cells decreased pH and osmolality due to metabolic activity. Proliferating cells showed no significant reaction to extracts, whereas differentiating cells were negatively influenced. In contrast to extract exposure, where cell size increased, in direct contact to magnesium, cell size was stable or even decreased. The amount of focal adhesions decreased over time on all materials. Genes involved in bone formation were significantly upregulated, especially for primary human osteoblasts. Some osteoinductive indicators were observed for OB: (i) an increased cell count after extract addition indicated a higher proliferation potential; (ii) increased cell sizes after extract supplementation in combination with augmented adhesion behavior of these cells suggest an early switch to differentiation; and (iii) bone-inducing gene expression patterns were determined for all analyzed conditions. The results from the cell lines were inhomogeneous and showed no specific stimulus of Mg. The comparison of the different cell types showed that primary cells of the investigated tissue should be used as an in vitro model if Mg is analyzed. (c) 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 165-179, 2017.
机译：基于镁的植入物表现出几种优点，例如生物降解性和可能的​​骨诱导性能。是否可以诱导新陈代谢的细胞类型特异性变化仍然不清楚。为了检查骨诱导性机制，测定骨衍生细胞（Mg63，U2OS，SAOS2和初级人骨细胞（OB））对镁（Mg）的反应。基于Mg的提取物用于模拟更现实的Mg降解条件。此外，研究了与降解Mg金属直接接触的细胞的影响。在暴露于提取物和直接接触中，由于代谢活性，细胞降低了pH和渗透压。增殖细胞显示出对提取物没有显着反应，而区分细胞受到负面影响。与提取曝光相反，其中细胞尺寸增加，直接接触镁，细胞尺寸稳定甚至降低。所有材料上的局灶性粘连量随时间减少。参与骨形成的基因显着上调，特别是对于原发性人骨细胞。对OB观察一些骨诱导指示剂：（i）提取物添加后的细胞计数增加表明增殖潜力较高; （ii）在提取补充后的细胞尺寸与这些细胞的增强粘合行为组合提取的细胞尺寸表明早期开关分化; （iii）确定所有分析的条件测定骨诱导的基因表达模式。细胞系的结果是不均匀的并且显示出Mg的特异性刺激。不同细胞类型的比较表明，如果分析Mg，则应使用所研究的组织的主要细胞作为体外模型。 （c）2015 Wiley期刊，Inc.J生物保解员B：Appl Biomater，105B：165-179,2017。

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