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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Tuning the response of osteoblast‐like cells to the porous‐alumina‐assisted mixed‐oxide nano‐mound arrays
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Tuning the response of osteoblast‐like cells to the porous‐alumina‐assisted mixed‐oxide nano‐mound arrays

机译：调节成骨细胞样细胞的响应，对多孔氧化铝辅助氧化物纳米土墩阵列

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Abstract Nanostructuring of biomaterials is used to create an appropriate interfacial layer that promotes stable cellular adhesion, proliferation, and differentiation on orthopedic and dental implants. Here, we synthesize self‐organized arrays of composite‐oxide nano‐mounds through anodizing Al/Ta bilayers sputtered on substrates to cover the “missing” smallest size range of 10–40 nm for structuring an advanced inorganic biomaterial—Al 2 O 3 ‐doped Ta 2 O 5 films. The osteoblast‐like cells appear to be able to recognize the finest differences in the film nano‐morphologies. In the absence of serum proteins, the adhesion and cell growth are substantially enhanced on the 20 and 40 nm nanoarrays while in complete medium the cells show better initial adhesion on the 10 nm nanoarrays. The proliferation assay reveals a significant rise in cell number on the 20 and 40 nm nanoarrays during the first 7 days. A remarkable increase in the alkaline phosphatase activity is noticed on the 40 nm nanoarray. Immunostaining of cells adhered to the nano‐mound surfaces shows that the cells are well spread over all the nanostructured films with organized actin fibers. The larger surface areas and improved focal contacts are again associated with the 20 and 40 nm nanoarrays. The findings help improve compatibility of living cells with the metal‐oxide nanostructured surfaces developed for tissue engineering. ? 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1645–1654, 2018.
机译：摘要生物材料的纳米结构用于产生适当的界面层，促进稳定的细胞粘附，增殖和牙科植入物上的分化。在此，我们通过在基材上溅射的阳极氧化Al / Ta双层来合成自组织的复合氧化物纳米土阵列，以覆盖用于构建先进无机生物材料-203的10-40nm的“缺失”最小尺寸范围。掺杂Ta 2 O 5薄膜。成骨细胞样细胞似乎能够识别膜纳米形态的最佳差异。在没有血清蛋白质的情况下，在20和40nm纳米阵列上基本上增强了粘附和细胞生长，而在完全培养基中，细胞在10nm纳米阵列上显示出更好的初始粘附性。增殖测定显示在前7天内20和40nm纳米阵列的细胞数显着上升。在40nm纳米阵列上注意到碱性磷酸酶活性的显着增加。粘附在纳米墩表面上的细胞的免疫染色表明细胞在所有纳米结构薄膜上均匀地展开，其中组织的肌动蛋白纤维。较大的表面积和改进的焦距接触再次与20和40nm纳米阵列相关联。结果有助于提高活细胞与为组织工程开发的金属氧化物纳米结构表面的相容性。还2017年Wiley期刊，Inc。J生物保罗部分B：Appl Biomater，106B：1645-1654,2018。

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