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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >In vitro In vitro and in vivo in vivo degradation of microfiber bioresorbable coronary scaffold
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In vitro In vitro and in vivo in vivo degradation of microfiber bioresorbable coronary scaffold

机译：体外体外和体内体内降解微纤维生物可吸血鬼冠状动道脚手架

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Abstract The degradation of Mirage Bioresorbable Microfiber Scaffold was evaluated in vitro and in vivo . The degradation in polymer molecular weight (MW), strut morphology, and integrity was accessed using gel permeation chromatography (GPC), X‐ray micro‐computed tomography (micro‐CT) evaluation. To simulate the physiological degradation in vitro , scaffolds were deployed in silicone mock vessels connected to a peristaltic pumping system, which pumps 37°C phosphate‐buffered saline (PBS, pH 7.4) at a constant rate. At various time points ( 30D, 60D, 90D, 180D, 270D, and 360D ), the MW of microfibers decreased to 57.3, 49.8, 36.9, 13.9, 6.4, and 5.1% against the baseline. The in vivo degradation study was performed by implanting scaffolds in internal thoracic arteries (ITAs) of mini‐swine. At the scheduled sacrifice time points (30D, 90D, 180D, 270D, 360D, and 540D), the implanted ITAs were excised for GPC analysis; the MW of the implanted scaffolds dropped to 58.5, 34.7, 24.8, 16.1, 12.9, and 7.1, respectively. Mass loss of scaffolds reached 72.4% at 540D of implantation. Two stages of hydrolysis were observed in in vitro and in vivo degradation kinetics, and the statistical analysis suggested a positive correlation between in vivo and in vitro degradation. After 6 months of incubation in animals, significant strut degradation was seen in the micro‐CT evaluation in all sections as strut fragments and separations. The micro‐CT results further confirmed that every sample at 720D had X‐ray transmission similar to surrounding tissue, thereby indicating full degradation within 2 years. ? 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1842–1850, 2018.
机译：摘要在体外和体内评估幻影生物可吸血剂微纤维支架的降解。使用凝胶渗透色谱（GPC），X射线微型计算机断层扫描（Micro-CT）评估，进入聚合物分子量（MW），Strut形态和完整性的降解。为了模拟体外的生理降解，在连接到蠕动泵送系统的硅氧烷模拟容器中部署了支架，其以恒定速率泵送37℃磷酸盐缓冲的盐水（PBS，pH7.4）。在各种时间点（30d，60d，90d，180d，270d和360d），微纤维的Mw降低至基线的57.3,49.8,36.9,13.9,6.4和5.1％。通过植入迷你猪的内部胸部动脉（ITA）中的支架进行体内降解研究。在预定的牺牲时间点（30D，90D，180D，270D，360D和540D）中，切除植入的ITA以进行GPC分析;植入支架的MW分别下降至58.5,34.7,24.8,16.1,12.9和7.1。 540D植入率的支架损失达到72.4％。在体外和体内降解动力学中观察到两种水解阶段，统计分析表明体内和体外降解之间的正相关性。在动物孵育6个月后，在所有部分中的微型CT评估中观察到显着的脱硫作为支柱片段和分离。微型CT结果进一步证实，720D时的每个样品具有类似于周围组织的X射线透射，从而在2年内表现出完全降解。还2017 Wiley期刊，Inc .J生物保解员B：Appl Biomater，106B：1842-1850,2018。

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