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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Three‐dimensional direct cell bioprinting for tissue engineering
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Three‐dimensional direct cell bioprinting for tissue engineering

机译：组织工程的三维直接细胞生物印刷

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摘要

Abstract Bioprinting is a relatively new technology where living cells with or without biomaterials are printed layer‐by‐layer in order to create three‐dimensional (3D) living structures. In this article, novel bioprinting methodologies are developed to fabricate 3D biological structures directly from computer models using live multicellular aggregates. Multicellular aggregates made out of at least two cell types from fibroblast, endothelial and smooth muscle cells are prepared and optimized. A novel bioprinting approach is proposed in order to continuously extrude cylindrical multicellular aggregates through the bioprinter's glass microcapillaries. The multicellular aggregates are first aspirated into a capillary and then compressed to form a continuous cylindrical multicellular bioink. To overcome surface tension‐driven droplet formation, the required compression ratio is calculated. Based on the developed bioprinting strategies, multicellular aggregates and their support structures are bioprinted to form 3D tissue constructs with predefined shapes. The effect of the bioprinting process was examined for fusion, cell viability at different compression ratios, and f‐actin cytoskeletal organization. The results show that the bioprinted 3D constructs fuse rapidly and have high cell viability after printing. ? 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2530–2544, 2017.
机译：摘要BioPlinting是一种相对较新的技术，其中具有或不含生物材料的活细胞被逐层印刷，以便创造三维（3D）活结构。在本文中，开发了新的生物制版方法，用于使用实时式多细胞聚集体直接从计算机模型制造3D生物学结构。制备和优化由成纤维细胞，内皮和平滑肌细胞中至少两种细胞类型制成的多细胞聚集体。提出了一种新的生物制版方法，以便通过Bioprinter的玻璃微涂料连续挤出圆柱形多细胞聚集体。首先将多细胞聚集体吸入毛细管中，然后压制以形成连续的圆柱形多细胞生物链。为了克服表面张力驱动的液滴形成，计算所需的压缩比。基于发达的生物监测策略，多细胞聚集体及其支撑结构是生物印刷，以形成具有预定形状的3D组织构造。对不同压缩比的融合，细胞活力和F-肌动蛋白细胞骨骼组织进行了融合的效果。结果表明，Bioplinted 3D在印刷后快速构建熔丝并具有高细胞活力。还2016 Wiley期刊，INC.J生物保解员B：苹果生物摩特，105B：2530-2544，2017。

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