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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Difference in suitable mechanical properties of three‐dimensional, synthetic scaffolds for self‐renewing mouse embryonic stem cells of different genetic backgrounds
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Difference in suitable mechanical properties of three‐dimensional, synthetic scaffolds for self‐renewing mouse embryonic stem cells of different genetic backgrounds

机译：三维合成支架的合适力学性能差异，用于不同遗传背景的自我更新小鼠胚胎干细胞

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Abstract We evaluated whether the genetic background of embryonic stem cells (ESCs) affects the properties suitable for three‐dimensional (3D) synthetic scaffolds for cell self‐renewal. Inbred R1 and hybrid B6D2F1 mouse ESC lines were cultured for 7 days in hydrogel scaffolds with different properties derived from conjugating 7.5, 10, 12.5, or 15% (wt/vol) vinylsulfone‐functionalized three‐, four‐, or eight‐arm polyethylene glycol (PEG) with dicysteine‐containing crosslinkers with an intervening matrix metalloproteinase‐specific cleavage sites. Cell proliferation and expression of self‐renewal‐related genes and proteins by ESCs cultured in feeder‐free or containing 2D culture plate or 3D hydrogel were monitored. As a preliminary experiment, the E14 ESC‐customized synthetic 3D microenvironment did not maintain self‐renewal of either the R1 or B6D2F1 ESCs. The best R1 cell proliferation (10.04 vs. 0.16–4.39; p ??0.0001) was observed in the four‐arm 7.5% PEG‐based hydrogels than those with other properties, whereas the F1 ESCs showed better proliferation when they were embedded in the three‐arm 10% hydrogels. Self‐renewal‐related gene and protein expression by ESCs after feeder‐free 3D culture was generally maintained compared with the feeder‐containing 2D culture, but expression patterns and quantities differed. However, the feeder‐free 3D culture yielded better expression than the feeder‐free 2D culture. In conclusion, genetic background determined the suitability of hydrogel scaffolds for self‐renewal of ESCs, which requires customization for the mechanical properties of each cell line. ? 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2261–2268, 2017.
机译：摘要我们评估了胚胎干细胞的遗传背景（ESC）是否影响了适用于三维（3D）合成支架的性能用于细胞自我更新。在水凝胶支架中培养近交R1和杂交B6D2F1小鼠ESC线，其具有不同的性质，其衍生自共轭7.5,10,12.5或15％（WT /体积）乙烯基砜 - 官能化的三 - ，四 - 或八臂聚乙烯乙二醇（PEG）与含有二丙氨酸的交联剂，具有介入基质金属蛋白酶酶的裂解位点。监测在无喂食器或含有2D培养板或3D水凝胶中培养的ESC的细胞增殖和自我更新相关基因和蛋白质的表达。作为初步实验，E14 ESC定制的合成3D微环境并未维持R1或B6D2F1 ESC的自我更新。在四臂7.5％PEG的水凝胶中观察到最佳的R1细胞增殖（10.04与0.16-4.39;p≤≤0.0001），而不是其他特性的水凝胶，而F1 ESC在嵌入时显示出更好的增殖在三臂10％水凝胶中。与含喂食器的2D培养物相比，通常保持自我更新相关的基因和蛋白质表达，但与含前的2D培养物相比，但表达模式和量不同。然而，无饲养的3D培养物比无饲养的2D培养物更好地表达。总之，遗传背景确定了水凝胶支架用于ESC的自我更新，这需要定制每种细胞系的机械性能。还2016 Wiley期刊，Inc .J生物保解率B部分B：苹果生物摩特，105B：2261-2268,2017。

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