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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Combining electrospinning and cell sheet technology for the development of a multiscale tissue engineered ligament construct (TELC)
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Combining electrospinning and cell sheet technology for the development of a multiscale tissue engineered ligament construct (TELC)

机译：相结合静电纺丝和细胞板技术来开发多尺度组织工程韧带构建体（TELC）

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摘要

Ligament tissue rupture is a common sport injury. Although current treatment modalities can achieve appropriate reconstruction of the damaged ligament, they present significant drawbacks, mostly related to reduced tissue availability and pain associated with tissue harvesting. Stem cell based tissue regeneration combined with electrospun scaffolds represents a novel treatment method for torn ligaments. In this study, a low fiber density polycaprolactone (PCL) electrospun mesh and sheep mesenchymal stem cells (sMSCs) were used to develop tissue engineered ligament construct (TELC) in vitro. The assembly of the TELC was based on the spontaneous capacity of the cells to organize themselves into a cell sheet once seeded onto the electrospun mesh. The cell sheet matured over 4 weeks and strongly integrated with the low fiber density electrospun mesh which was subsequently processed into a ligament-like bundle and braided with two other bundles to develop the final construct. Live/dead assay revealed that the handling of the construct through the various phases of assembly did not cause significant difference in viability compared to the control. Mechanical evaluation demonstrated that the incorporation of the cell sheet into the braided construct resulted in significantly modifying the mechanical behavior. A stress/displacement J-curve was observed for the TELC that was similar to native ligament, whereas this particular feature was not observed in the non-cellularized specimens. The regenerative potential of the TELC was evaluated ectopically in immunocompromized rats, compared to non cellularized electrospun fiber mesh and this demonstrated that the TELC was well colonized by host cells and that a significant remodelling of the implanted construct was observed. (c) 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 399-409, 2018.
机译：韧带组织破裂是一种常见的运动损伤。尽管目前的治疗方式可以实现适当的重建韧带，但它们存在显着的缺点，大多数与减少组织可用性和与组织收割相关的疼痛相关。基于干细胞基组织再生与Electurpum支架结合代表撕裂韧带的新型处理方法。在该研究中，使用低纤维密度聚己内酯（PCL）电纺网和绵羊间充质干细胞（SMSCs）在体外开发组织工程韧带构建体（TELC）。 Telc的组装基于细胞的自发性容量，以将自己组织成一旦接种到电纺网上的细胞片。细胞片在4周内成熟并与低纤维密度电纺丝啮合强烈集成，随后将其加工成韧带状束并用另外两个束编织以开发最终构建体。 Live / Dead测定显示，与控制相比，通过组装各个阶段处理构建体的处理没有造成显着差异。机械评估证明将细胞片掺入编织构建体中，导致显着改变机械行为。对于类似于本地韧带的TELC，观察到应力/位移J-曲线，而在非细胞化样本中未观察到该特定特征。与非细胞化电纺纤维网格相比，不同地评估Telc的再生潜力，并且与非细胞化的电纺纤维网相比，这证明了通过宿主细胞进行良好的沉淀，并且观察到植入构建体的显着重塑。 （c）2017年Wiley期刊，Inc。J生物保解员B：Appl Biomater，106B：399-409,2018。

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