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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Interaction of HEPES buffer with glass-ceramic scaffold: Can HEPES replace TRIS in SBF?
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Interaction of HEPES buffer with glass-ceramic scaffold: Can HEPES replace TRIS in SBF?

机译：HEPES缓冲液与玻璃陶瓷脚手架的相互作用：HEPES可以在SBF中替换TRIS吗？

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An international standard (ISO: 23317:2014) exists for the in vitro testing of inorganic biomaterials in simulated body fluid (SBF). This standard uses TRIS buffer to maintain neutral pH in SBF, but in our previous paper, we showed that the interaction of a tested glass-ceramic material with TRIS can produce false-positive results. In this study, we evaluated whether the HEPES buffer, which also belongs to the group of Good ' s buffers, would be more suitable for SBF. We compared its suitability in two media: SBF with HEPES and demineralized water with HEPES. The tested scaffold (45S5 bioactive glass-based) was exposed to the media under a static-dynamic arrangement (solutions were replaced on a daily basis) for 15 days. Leachate samples were collected daily for the analysis of Ca2+ ions and Si (AAS), (PO4)(3-) ions (UV-VIS), and to measure pH. The glass-ceramic scaffold was analyzed by SEM/EDS, XRD, and WD-XRF before and after 0.3, 1, 3, 7, 11, and 15 days of exposure. Our results confirmed the rapid selective dissolution of the glass-ceramic crystalline phase (Combeite) containing Ca2+ ions due to the presence of HEPES, hydroxyapatite supersaturation being reached within 24 h in both solutions. These new results suggest that, like TRIS, HEPES buffer is not suitable for the in vitro testing of highly reactive inorganic biomaterials (glass, glass-ceramics). The ISO standard for such tests requires revision, but HEPES is not a viable alternative to TRIS buffer. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 143-152, 2018.
机译：存在国际标准（ISO：23317：2014），用于模拟体液（SBF）中无机生物材料的体外测试。本标准使用TRIS缓冲液在SBF中保持中性pH，但在我们之前的纸张中，我们表明，测试的玻璃陶瓷材料与TRIS的相互作用可以产生假阳性结果。在这项研究中，我们评估了HEPES缓冲区是否属于良好的缓冲区的群体，更适合SBF。我们将其适用于两种媒体：SBF与HEPES和脱矿水的水。在静动系布置下将测试的支架（45S5生物活性玻璃基）暴露于培养基（每天替换溶液）15天。每天收集渗滤液样品，用于分析Ca2 +离子和Si（AAS），（PO4）（3-）离子（UV-Vis），并测量pH值。通过SEM / EDS，XRD和WD-XRF分析玻璃陶瓷支架，然后在0.3,1,3,7,11和15天后进行的。我们的结果证实，由于HEPES存在，含有Ca2 +离子的玻璃陶瓷结晶相（组合）的快速选择性溶解，羟基磷灰石过饱和在两种溶液中的24小时内达到。这些新结果表明，与TRI一样，HEPES缓冲液不适用于高反应性无机生物材料的体外测试（玻璃，玻璃陶瓷）。此类测试的ISO标准需要修改，但HEPES不是TRIS缓冲区的可行替代品。 （c）2016年Wiley期刊，Inc。J生物保解员B部分B：苹果生物摩特，106B：143-152,2018。

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