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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Development of a chitosan-based tissue-engineered renal proximal tubule conduit
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Development of a chitosan-based tissue-engineered renal proximal tubule conduit

机译：基于壳聚糖的组织工程肾近端小管导管的开发

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Renal proximal tubule cells (RPTCs) are responsible for glomerular filtration and maintenance of water/electrolyte balance. To regenerate a proximal tubule, sufficient cell numbers and normal cell function are requisite. Collagen has been routinely used as a substrate for culturing human RPTCs (HRPTCs); however, the role of biomaterials has not been thoroughly explored. In this study, RPTCs retrieved from human nephrectomy/nephroureterectomy specimens were cultivated on chitosan as a substrate in serum-free condition for up to 150 days. HRPTCs could maintain a typical epithelial morphology and the specific differentiation feature of transporting epithelia after such long-term culture. As compared with HRPTCs cultivated on collagen, those cultivated on chitosan showed more dome formation, higher Na+-K+ ATPase expression, lower vimentin expression, and lower transepithelial electrical resistance, indicating that HRPTCs cultivated on chitosan presented better differentiation status and would be more functional with better active transportation. Thus, the current study indicates greater scope for the use of chitosan as a biomaterial for preparing a HRPTC-coated chitosan conduit, which might be useful for the scaffold design of tissue-engineered proximal tubules. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 9-20, 2018.
机译：肾近端小管细胞（RPTC）负责肾小球过滤和水/电解质平衡的维持。为了再生近端小管，需要足够的细胞数和正常电池功能是必需的。胶原蛋白经常用作培养人RPTC（HRPTC）的基材;然而，生物材料的作用尚未彻底探索。在本研究中，从人肾病术/肾病术标本检索的RPTC在壳聚糖上培养，作为血清条件的底物，最多150天。 HRPTCs可以保持典型的上皮形态和在这种长期培养后运输上皮细胞的特定分化特征。与胶原蛋白栽培的HRPTC相比，壳聚糖栽培的人培养了更多圆顶形成，较高的Na + -K + ATP酶表达，降低的植物表达和降低的培壳多糖培养的HRPTC呈现出更好的分化状态，并将更加功能更好的积极运输。因此，目前的研究表明使用壳聚糖作为制备HRPTC涂覆的壳聚糖导管的生物材料的使用范围，这可能对组织工程近端小管的支架设计有用。 （c）2016 Wiley期刊，Inc.J生物保解员B：Appl Biomater，106B：9-20,2018。

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