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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Osteogenic potential of stem cells-seeded bioactive nanocomposite scaffolds: A comparative study between human mesenchymal stem cells derived from bone, umbilical cord Wharton's jelly, and adipose tissue
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Osteogenic potential of stem cells-seeded bioactive nanocomposite scaffolds: A comparative study between human mesenchymal stem cells derived from bone, umbilical cord Wharton's jelly, and adipose tissue

机译：干细胞种子生物活性纳米复合支架的成骨潜力潜力：骨髓，脐带涡轮果冻和脂肪组织的人间充质干细胞的对比研究

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摘要

Bone regeneration is considered as an unmet clinical need, the aim of this study is to investigate the osteogenic potential of three different mesenchymal stem cells (MSCs) derived from human bone marrow (BM-MSCs), umbilical cord Wharton's jelly (UC-MSCs), and adipose (AD-MSCs) seeded on a recently developed nanocomposite scaffold (bioactive glass/gelatin) implanted in rat animal models with critical size calvarial defects. In this study, after isolation, culture, and characterization, the MSCs were expanded and seeded on the scaffolds for in vitro and in vivo studies. The adhesion, proliferation, and viability of the cells on the scaffolds evaluated in vitro, showed that the scaffolds were biocompatible for further examinations. In order to evaluate the scaffolds in vivo, rat animal models with critical size calvarial defects were randomly categorized in four groups and treated with the scaffolds. The animals were sacrificed at the time points of 4 and 12 weeks of post-implantation, bone healing process were investigated. The histological and immunohistological observations showed (p 0.01) higher osteogenesis capacity in the group treated with BM-MSCs/scaffolds compared to the other groups. However, the formation of new angiogenesis was evidently higher in the defects filled with UC-MSCs/scaffolds. This preliminary study provides promising data for further clinical trials. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 61-72, 2018.
机译：骨再生被认为是未核心的临床需求，本研究的目的是研究来自人骨髓（BM-MSCs），脐带涡轮果冻（UC-MSCs）的三种不同间充质干细胞（MSCs）的成骨潜力并且在最近开发的纳米复合支架（生物活性玻璃/明胶）上植入大鼠动物模型的脂肪（Ad-MSCs），具有临界大小的颅骨缺陷。在本研究中，在分离，培养和表征之后，MSCs在体外和体内研究中膨胀和接种在支架上。在体外评估的支架上的细胞对细胞的粘附，增殖和活力表明，用于进一步检查的支架是生物相容性的。为了评估体内的支架，大鼠动物模型具有临界大小的颅骨缺陷被随机分类为四组，并用支架处理。在植入后4和12周的时间点处死动物，研究了骨愈合过程。与其他基团相比，组织学和免疫组织学观察显示（P <0.01）的骨质发生能力较高。然而，在充满UC-MSCs /支架的缺陷中，新血管生成的形成显然较高。这项初步研究提供了进一步临床试验的有希望的数据。 （c）2016 Wiley期刊，Inc.J生物保解员B：Appl Biomater，106B：61-72,2018。

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