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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >A two‐step method using air plasma and carbodiimide crosslinking to enhance the biocompatibility of polycaprolactone
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A two‐step method using air plasma and carbodiimide crosslinking to enhance the biocompatibility of polycaprolactone

机译：一种两步法，采用空气等离子体和碳二亚胺交联以增强聚己内酯的生物相容性

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Abstract In this study, polycaprolactone (PCL) film, a high potential material used in biomedical applications, was treated by air plasma prior to a conjugation by carbodiimide cross‐linking with various types of proteins, including type A gelatin, type B gelatin, and collagen hydrolysate. The properties of modified PCL films were characterized by X‐ray photoelectron spectroscopy (XPS), contact angle measurement, and atomic force microscopy. The XPS results showed that oxygen and nitrogen atoms were successfully introduced on the air plasma‐treated PCL surface. Primary amine was found on the air plasma‐treated PCL films. All proteins were shown to be successfully cross‐linked on air plasma‐treated PCL films. The wettability and roughness of protein‐conjugated PCL films were significantly increased compared to those of neat PCL film. In vitro biocompatibility test using L929 mouse fibroblast showed that the attachment percentage and spreading area of attached cells on all protein‐conjugated PCL films were markedly increased. Comparing among modified PCL films, no significant difference on the attachment of L929 on modified PCL films was noticed. However, the spreading areas of cells after 24?hours of culture on type A gelatin‐ and type B gelatin‐modified PCL surfaces were higher than that on collagen hydrolysate‐modified surface, possibly related to the lower percentage of amide bond on collagen hydrolysate‐conjugated surface compared to those on both gelatin‐conjugated PCL ones. This indicated that the two‐step modification of PCL film via air plasma and carbodiimide cross‐linking with collagen‐derived proteins could enhance the biocompatibility of PCL films. ? 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1658–1666, 2017.
机译：摘要在本研究中，通过碳二亚胺交联在与各种类型的蛋白质的交联之前，通过空气等离子体处理聚己内酯（PCL）膜，生物医学应用中使用的高潜在材料，包括型蛋白质，包括型明胶，B型明胶，和胶原蛋白水解物。通过X射线光电子能谱（XPS），接触角测量和原子力显微镜表征改性PCL膜的性质。 XPS结果表明，在空气等离子体处理的PCL表面上成功地引入了氧气和氮原子。在空气等离子体处理的PCL薄膜上发现伯胺。显示所有蛋白质在空气等离子体处理的PCL薄膜上成功交联。与整齐的PCL薄膜相比，蛋白质共轭PCL薄膜的润湿性和粗糙度显着增加。使用L929小鼠成纤维细胞的体外生物相容性试验表明，所有蛋白质共轭PCL薄膜上的附着百分比和附着细胞的扩散区域明显增加。在改性的PCL膜中比较，注意到L929的附着在改性的PCL薄膜上没有显着差异。然而，24小时后细胞的蔓延区域在型明胶和B型明胶改性的PCL表面上高于胶原水解改性表面上的培养物，可能与胶原水解酯酸盐酯酯的较低百分比有关 - 与明胶缀合的PCL组上的缀合表面相比。这表明通过空气血浆和碳二亚胺与胶原衍生蛋白交联的PCL膜的两步改变可以增强PCL薄膜的生物相容性。还2016 Wiley期刊，Inc。J生物母乳部分B：Appl Biomater，105B：1658-1666,2017。

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