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首页> 外文期刊>Journal of biomedical materials research. Part B, Applied biomaterials. >Cartilage/bone interface fabricated under perfusion: Spatially organized commitment of adipose-derived stem cells without medium supplementation
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Cartilage/bone interface fabricated under perfusion: Spatially organized commitment of adipose-derived stem cells without medium supplementation

机译：在灌注下制造的软骨/骨界面：没有介质补充的脂肪衍生的干细胞的空间有组织的承诺

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Tissue engineering of an osteochondral interface demands for a gradual transition of chondrocyte- to osteoblast-prevailing tissue. If stem cells are used as a single cell source, an appropriate cue to trigger the desired differentiation is the use of composite materials with different amounts of calcium phosphate. Electrospun meshes of poly-lactic-co-glycolic acid and amorphous calcium phosphate nanoparticles (PLGA/aCaP) in weight ratios of 100:0; 90:10, 80:20, and 70:30 were seeded with human adipose-derived stem cells (ASCs) and cultured in DMEM without chemical supplementation. After 2 weeks of static cultivation, they were either further cultivated statically for another 2 weeks (group 1), or placed in a Bose (R) bioreactor with a flow rate per area of 0.16 mL cm(-2) min(-1) (group 2). Markers for stem cell criteria, chondrogenesis, osteogenesis, adipogenesis and angiogenesis were analyzed by quantitative real-time PCR. Cell distribution, Sox9 protein expression and proteoglycans were assessed by histology. In group 2 (perfusion culture), chondrogenic Sox9 was upregulated toward the cartilage-mimicking side compared to pure PLGA. On the bone-mimicking side, Sox9 experienced a downregulation, which was confirmed on the protein level. Vice versa, expression of osteocalcin was upregulated on the bone-mimicking side, while it was unchanged on the cartilage-mimicking side. In group 1 (static culture), CD31 was upregulated in the presence of aCaP compared to pure PLGA, whereas Sox9 and osteocalcin expression were not affected. aCaP nanoparticles incorporated in electrospun PLGA drive the differentiation behavior of human ASCs in a dose-dependent manner. Discrete gradients of aCaP may act as promising osteochondral interfaces. (C) 2018 Wiley Periodicals, Inc.
机译：骨质色神经界面的组织工程要求软骨细胞逐渐过渡到截止卵泡血液细胞的逐步转变。如果干细胞用作单个细胞源，则触发所需分化的适当提示是使用具有不同量的磷酸钙的复合材料。电纺成型的聚乳酸 - 共乙酸和磷酸钙纳米粒子（PLGA / ACAP）的重量比为100：0; 90:10，80:20和70:30与人脂肪衍生的干细胞（ASCS）播种，并在DMEM中培养而不进行化学补充。经过2周的静态培养后，它们在静态静态培养2周（第1组），或置于培养（R）生物反应器中，流速为0.16ml cm（-2）min（-1） （第2组）。通过定量的实时PCR分析干细胞标准，软骨发生，骨发生，脂肪发生和血管生成的标记。通过组织学评估细胞分布，SOX9蛋白表达和蛋白转基因组合体。在第2组（灌注培养物）中，与纯PLGA相比，朝向软骨模拟侧上调有软骨菌SOx9。在骨模拟侧，SOX9经历了下调，在蛋白质水平上证实。反之亦然，骨钙素的表达在骨模拟侧上调，而在软骨模拟侧不变。在第1组（静态培养）中，与纯PLGA相比，CD31在ACAP的存在下进行上调，而SOX9和骨钙素表达不受影响。掺入Electrom淘PLGA的ACAP纳米颗粒以剂量依赖性方式驱动人ASC的分化行为。 ACAP的离散梯度可能充当承诺的骨削接口。 （c）2018 Wiley期刊，Inc。

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