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Production of a sialylated N-linked glycoprotein in insect cells

机译：在昆虫细胞中产生唾液酸化的N连接糖蛋白

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摘要

Under High Aspect Ratio Vessel (HARV) bioreactor culture conditions designed to simulate the microgravity of orbital space flight, insect tissue culture cells infected with a baculovirus expression vector produced a human glycoprotein with tri- and tetra-antennary complex N-linked oligosaccharides containing terminal sialic acid residues. The oligosaccharide structures were similar to those produced in human placental cells. Insect cells cultured in T-flasks only performed incomplete oligosaccharide processing. The mechanism of HARV-mediated changes in the eukaryotic N-linked glycosylation pathway was investigated and could be mimicked under T-flask growth conditions with the addition of N-acetylmannosamine to the culture medium. The significance of these investigations is discussed with respect to the production of recombinant therapeutic glycoproteins, insect physiology, and orbital space flight.
机译：在旨在模拟轨道太空飞行的微重力的高纵横比容器（HARV）生物反应器培养条件下，感染杆状病毒表达载体的昆虫组织培养细胞产生了人类糖蛋白，该蛋白带有三末端和四末端复杂的N末端寡唾液酸N联寡糖酸残基。寡糖结构与人胎盘细胞产生的寡糖结构相似。在T瓶中培养的昆虫细胞仅进行了不完全的低聚糖加工。研究了HARV介导的真核N-连接糖基化途径变化的机制，可以在T瓶生长条件下向培养基中添加N-乙酰甘露糖胺来模拟HARV介导的变化。讨论了这些研究的意义，涉及重组治疗性糖蛋白的产生，昆虫生理学和轨道太空飞行。

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