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首页> 外文期刊>Biotechnology Progress >Optimization of conditions for bacteriocin extraction in PEG/salt aqueoustwo-phase systems using statistical experimental designs
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Optimization of conditions for bacteriocin extraction in PEG/salt aqueoustwo-phase systems using statistical experimental designs

机译：使用统计实验设计优化PEG /盐水溶液两相系统中细菌素提取的条件

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摘要

The bacteriocin nisin was extracted in PEG/salt aqueous two-phase systems (ATPS) using the property that the systems can extract hydrophobic proteins. The concentrations of the phase-forming components, PEG 4000 and Na2SO4, were optimized for nisin recovery by means of statistical experimental designs, and it was found that they strongly influenced nisin recovery. The optimal composition of ATPS was found to be 15.99% (w/w) PEG 4000 and 15.85% (w/w) Na2SO4 (pH 2), and the optimal ATPS allowed an 11.60% increase of nisin recovery compared to the standard method of nisin assay.
机译：利用PEG /盐水溶液两相系统（ATPS）可以提取疏水蛋白的特性，提取细菌素乳链菌肽。通过统计实验设计，优化了相形成成分PEG 4000和Na2SO4的浓度以用于乳链菌肽的回收，发现它们强烈影响乳链菌肽的回收。发现ATPS的最佳组成为15.99％（w / w）PEG 4000和15.85％（w / w）Na2SO4（pH 2），与标准方法相比，最佳ATPS可使乳酸链球菌素回收率提高11.60％。乳链菌肽测定。

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