• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology Progress >Expanded application of a two-phase partitioning bioreactor through strain development and new feeding strategies
【24h】

Expanded application of a two-phase partitioning bioreactor through strain development and new feeding strategies

机译：通过菌株开发和新的进料策略扩大了两相分配生物反应器的应用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This research demonsrated the microbial treatment of concentrated phenol wastes using a two-phase partitioning bioreactioning bioreactor (TPPB).TPPBs are characterized by a cellcontaining aqueous phase and an immiscible and biocompatible organic phase that partitions toxic substrates to the cells on the basis of their metabolic demand and the thermodynamic equilibrium of the system.Process limitations imposed by the capability of wild-type Pseudomonas Pputida ATCC 11172 to utilize long chain alcohols were addressed by strain modification(transposon mutagenesis) toeliminate this undesirable biochemical characteristic,enabling use of a range of previously bioavailable organics as delivery solvents.Degradation of phenol in a system with the modified strain as catalyst and industrial grade Adol 85 NF(primarily oleyl alcohol) as the solvent was demonstrated,with the system ultimately degrading 36 of phenol within 38h.Volumetric phenol consumption rates by wild type P.putida ATCC 11172 and the genetically modified derivative revealed equivalent phenol degrading capabilities (0.49 g/Lcentre doth respectively,in paired fermentations),with the latter presenting a more efficient remediation option due to decreased solvent losses arising from the modified strain's forced inability to consume the delivery solvent as a substrate.Two feeding strategies and system configurations were evaluated to expand practical applications of TPPB technology.The ability to operate with a lower solvent ratio over extended periods revealed potential for long-termapplication of TPPB to the treatment of large masses of phenol while minimizing solvent costs.Repeated recovery of 99% of phenolformconcentratedpenolsolutions and subsequent treatment within a TPB scheme demonstrated applicability of the approach to the remediationof highly contaminated "effluents"as well as large masses of bulk phenol.Operationof the TPPB system in a dispersed amnner,rather thanas two distinct phases,resulted in volumetric consumption rates similar to those previously achieved onlyin systems operated with enriched air.
机译：这项研究通过使用两相分配生物反应生物反应器（TPPB）去除了微生物处理浓苯酚废物的特征.TPPBs的特征是含有细胞的水相和不溶混且具有生物相容性的有机相，可根据新陈代谢将有毒底物分配给细胞通过菌株修饰（转座子诱变）解决了野生型假单胞菌恶臭假单胞菌ATCC 11172利用长链醇的能力所施加的过程限制，以消除这种不良的生化特性，从而可以使用一系列以前的方法。以改性菌株为催化剂，工业级Adol 85 NF（主要为油醇）为溶剂的体系中的苯酚降解，该体系最终在38h内降解了36种苯酚。按野生型恶臭假单胞菌ATCC 11172和t转基因衍生物显示出等效的苯酚降解能力（成对发酵中分别为0.49 g / Lcentre doth），由于改性菌株被迫无法消耗递送溶剂作为溶剂而导致的溶剂损失减少，后者表现出更有效的修复选择。对两种进料策略和系统配置进行了评估，以扩展TPPB技术的实际应用。在较低的溶剂比下长期运行的能力表明，TPPB可以长期用于处理大量苯酚，同时将溶剂成本降至最低，具有长期应用潜力重复回收99％浓缩的苯酚形式的苯酚溶液，并在TPB方案中进行后续处理，证明该方法适用于修复高污染的“废水”以及大量的散装苯酚。TPPB系统在分散的容器中操作，而不是两个不同的操作相，以体积计ic消耗率类似于以前仅在使用富空气的系统中实现的消耗率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号