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首页> 外文期刊>Journal of Biophotonics >Multimodal imaging to explore endogenous fluorescence of fresh and fixed human healthy and tumor brain tissues
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Multimodal imaging to explore endogenous fluorescence of fresh and fixed human healthy and tumor brain tissues

机译：探讨新鲜和固定人类健康和肿瘤脑组织内源性荧光的多峰成像

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>To complement a project toward label‐free optical biopsy and enhanced resection which the overall goal is to develop a multimodal nonlinear endomicroscope, this multimodal approach aims to enhance the accuracy in classifying brain tissue into solid tumor, infiltration and normal tissue intraoperatively. Multiple optical measurements based on one‐ and two‐photon spectral and lifetime autofluorescence, including second harmonic generation imaging, were acquired. As a prerequisite, studying the effect of the time of measurement postexcision on tissue's spectral/lifetime fluorescence properties was warranted, so spectral and lifetime fluorescences of fresh brain tissues were measured using a point‐based linear endoscope. Additionally, a comparative study on tissue's optical properties obtained by multimodal nonlinear optical imaging microscope from fresh and fixed tissue was necessary to test whether clinical validation of the nonlinear endomicroscope is feasible by extracting optical signatures from fixed tissue rather than from freshly excised samples. The former is generally chosen for convenience. Results of this study suggest that an hour is necessary postexcision to have consistent fluorescence intensitieslifetimes. The fresh (a,b,c) vs fixed (d,e,f) tissue study indicates that while all optical signals differ after fixation. The characteristic features extracted from one‐ and two‐photon excitation still discriminate normal brain (a,d) cortical tissue, glioblastoma (GBM) (b,e) and metastases (c,f).
机译： >将项目补充到无标记的光学活检和增强的切除，整体目标是开发多峰非线性计时器腔体，这种多峰方法旨在提高脑内分类脑组织的准确性，术上肿瘤，渗透和正常组织。获取基于单光子谱和寿命自发的多种光学测量，包括二次谐波生成成像，包括二次谐波生成成像。作为先决条件，有保证测量时间效果对组织的光谱/寿命荧光性质的影响，因此使用基于点的线性内窥镜测量新鲜脑组织的光谱和寿命荧光。另外，需要对新鲜和固定组织获得的多模式非线性光学成像显微镜获得的组织光学性质的比较研究是为了测试非线性内瘤的临床验证是通过从固定组织而不是新切除的样品中提取光学签名来可行。前者通常为方便起见。该研究的结果表明，一小时是必要的荧光强度寿命。新鲜（a，b，c）的Vs固定（d，e，f）组织研究表明，虽然所有光学信号在固定后均不同。从单光子激发中提取的特征仍然区分正常的脑（A，D）皮质组织，胶质母细胞瘤（GBM）（B，E）和转移（C，F）。

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