Improvement of enzyme stability for alkyl esters synthesis in miniemulsion systems by using media engineering

de Barros Dragana PC; Pinto Fátima; Pfluck Ana CD; Dias Andreia SA; Fernandes Pedro; Fonseca Luís P

摘要

Abstract <section xml:id="jctb5499-sec-0001" numbered="no"> <title type="main">BACKGROUND > Oil‐in‐water miniemulsions have been suggested to display potential for application as a ‘green’ system for enzymatic alkyl ester synthesis. Still, a realistic assessment of the feasibility of this approach for practical applications requires further insight, namely the relation between enzyme stability and reaction operational conditions. The objective of this work is within such scope. Accordingly, it extends previous research on esterification catalyzed by <fi>Fusarium solani pisi</fi> cutinase, by addressing medium‐chain length substrates, and aims to contribute to the optimization of reaction conditions and towards the design of an efficient set‐up. </section> <section xml:id="jctb5499-sec-0002" numbered="no"> <title type="main">RESULTS > The esterification yield was significantly enhanced (e.g. 50% increase for octyl octanoate system) through the correction of the initial pH of the reaction medium to pH 6. Under such environment, conformational changes of the enzyme were negligible after 24 h, resulting in improved enzyme stability for all systems under study. Maximum esterification rate (3.59 mmol min ‐1 ) and product yield (91%) were achieved for octyl decanoate synthesis. Fed‐batch mode of operation allowed for the production of 1 mol L ‐1 hexyl octanoate, while retaining enzyme activity over 5 days. Consecutive batch runs allowed for the cumulative production of 1.1 mol L ‐1 hexyl octanoate after four cycles. </section> <section xml:id="jctb5499-sec-0003" numbered="no"> <titl </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“JCTB5499-SEC-0001”编号=“否”> <标题类型=“main”>背景</标题> >水含油小型乳液，以显示应用作为“绿色”系统的应用用于酶促烷基酯合成。尽管如此，对实际应用方法的可行性的实际评估需要进一步的见解，即酶稳定性与反应操作条件之间的关系。这项工作的目标是在这样的范围内。因此，通过寻址中链长度基材，旨在通过寻址中链长度底物催化的酯化催化的酯化研究，并旨在有助于优化反应条件和设计有效设置的设计。 </ p> </ section> <section XML：ID =“JCTB5499-SEC-0002”编号=“否”> <标题类型=“main”>结果</标题> >酯化产量显着增强（例如，辛基辛酸系统的50％增加了辛基酸盐系统）通过将反应介质的初始pH校正至pH6。在这种环境下，24小时后，酶的构象变化可以忽略不计，导致所有系统的酶稳定性改善。对于辛基癸酸盐合成，实现了最大酯化速率（3.59mmol min -1℃）和产物产率（91％）。允许批量操作模式允许生产1mol L -1 -1 / sop>羟基丙酯，同时保持酶活性超过5天。连续批量运行允许累计生产1.1 mol l -1 -1 / sup>己基后四个循环后奥替纳酸盐。 </ p> </ section> <第XML：ID =“JCTB5499-SEC-0003”编号=“否”> <titl </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第5期</span><b style="margin: 0 2px;">|</b><span>共9页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=de Barros Dragana PC&option=202" target="_blank" rel="nofollow">de Barros Dragana PC;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pinto Fátima&option=202" target="_blank" rel="nofollow">Pinto Fátima;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pfluck Ana CD&option=202" target="_blank" rel="nofollow">Pfluck Ana CD;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Dias Andreia SA&option=202" target="_blank" rel="nofollow">Dias Andreia SA;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fernandes Pedro&option=202" target="_blank" rel="nofollow">Fernandes Pedro;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fonseca Luís P&option=202" target="_blank" rel="nofollow">Fonseca Luís P;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of Bioengineering and IBB – Institute for Bioengineering and BiosciencesUniversidade de LisboaLisboa Portugal;</p> <p>Department of Bioengineering and IBB – Institute for Bioengineering and BiosciencesUniversidade de LisboaLisboa Portugal;</p> <p>Department of Bioengineering and IBB – Institute for Bioengineering and BiosciencesUniversidade de LisboaLisboa Portugal;</p> <p>Department of Bioengineering and IBB – Institute for Bioengineering and BiosciencesUniversidade de LisboaLisboa Portugal;</p> <p>Department of Bioengineering and IBB – Institute for Bioengineering and BiosciencesUniversidade de LisboaLisboa Portugal;</p> <p>Department of Bioengineering and IBB – Institute for Bioengineering and BiosciencesUniversidade de LisboaLisboa Portugal;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=alkyl esters&option=203" rel="nofollow">alkyl esters;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fusarium solani pisi cutinase&option=203" rel="nofollow">Fusarium solani pisi cutinase;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=miniemulsion systems&option=203" rel="nofollow">miniemulsion systems;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=operational stability&option=203" rel="nofollow">operational stability;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=enzyme reutilization&option=203" rel="nofollow">enzyme reutilization;</a> </p> <div class="translation"> 机译：烷基酯;镰刀菌索尔甘油pisi culinase;迷你乳液系统;操作稳定性;酶再利用; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422849.html">Improvement of enzyme stability for alkyl esters synthesis in miniemulsion systems by using media engineering</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=de Barros Dragana PC&option=202" 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href="/academic-journal-cn_journal-ludong-university-natural-science-edition_thesis/0201270210636.html">利用聚4-乙烯基吡啶改进微乳液凝胶中脂肪酶的稳定性</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=黄玉竹&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 黄玉竹</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=陶倩&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,陶倩</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李桂英&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李桂英</a> <span> <a href="/journal-cn-4528/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 鲁东大学学报（自然科学版） </a> </span> <span> . 2019</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chemical-journal-chinese-universities_thesis/0201231622795.html">微乳液中脂肪酶和含明胶的微乳液凝胶中固定化脂肪酶的催化特性</a> <b>[J]</b> <span> 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</b><a class="enjiyixqcontent" href="/patent-detail/06120100016864.html">锰型高稳定性超氧化物歧化酶在改善自闭症中的应用及产品</a> <b>[P]</b> . <span> 中国专利： CN109276711A </span> <span> . 2019-01-29</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130415076026.html">AN EFFICIENT AND GREENER ENZYME CATALYZED METHODOLOGY FOR THE SYNTHESIS OF 2-PROPENOIC ACID-3-PHENYL-ALKYL ESTER COMPOUNDS IN SUPERCRITICAL CARBON DIOXIDE MEDIA</a> <b>[P]</b> . <span> 外国专利：  IN3172MU2015A </span> <span> . 2015-09-11</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：在超临界二氧化碳介质中合成2-戊酸-3-苯基烷基酯化合物的高效，绿色酶催化方法 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130468827656.html">synthesis of alkyl esters of carboxylic acid pentan - useful as intermediate in the synthesis of prostaglandins</a> <b>[P]</b> . <span> 外国专利：  IT1194245B </span> <span> . 1988-09-14</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：羧酸戊烷的烷基酯的合成-用作前列腺素合成的中间体 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130475398484.html">synthesis of alkyl esters of carboxylic acid pentan - useful as intermediate in the synthesis of prostaglandins.</a> <b>[P]</b> . <span> 外国专利：  IT8321145D0 </span> <span> . 1983-05-18</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：羧酸戊烷的烷基酯的合成-用作前列腺素合成的中间体。 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img 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