Design of a heterogeneous enzymatic catalyst on chitosan: investigation of the role of conjugation chemistry in the catalytic activity of a Laccase from <fi xmlns='http://www.wiley.com/namespaces/wiley'>Trametes versicolor</fi>Trametes versicolor

Apriceno Azzurra; Girelli Anna Maria; Scuto Francesca Romana

摘要

Abstract <section xml:id="jctb5509-sec-0001" numbered="no"> <title type="main">BACKGROUND > Three protocols are presented in order to immobilise a laccase (EC 1.10.3.2) from <fi>Trametes versicolor</fi> on chitosan. In particular, chitosan is functionalized with glutaraldehyde and epichlorohydrin to explore to what extent the conjugation of the enzyme is affected by a carbonyl‐ or epoxy‐modified surface, respectively. In addition, an oxidation procedure is tested to modify, for the first time, the carbohydrate moiety of the enzyme and exploit it for linking with the amine group of chitosan. </section> <section xml:id="jctb5509-sec-0002" numbered="no"> <title type="main">RESULTS >The system in which laccase is directly conjugated to chitosan seems to be the best‐performing since it is able to maintain 100% of initial activity over a 30 day period and at least 50% of the starting activity after 3 catalytic cycles. </section> <section xml:id="jctb5509-sec-0003" numbered="no"> <title type="main">CONCLUSION >The methods show the capability to develop three biocatalysts suitable for the immobilization of laccase. All of them provide covalent bonds that don't inactivate the enzyme through any conformational change and any distortion of the active pocket. Specifically, the oxidation procedure proves to be feasible for the preparation of stabilized glycoproteins without the introduction of foreign molecules, suggesting that the carbohydrate moiety of laccase does not appear to be closely related to the catalytic site of the protein. ? 2017 Society of Chemical Industry </section> </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract XMLNS =“http://www.wiley.com/namespaces/wiley”type =“main”xml：lang =“en”> <title type =“main”>抽象</ title> <section XML：ID =“JCTB5509-SEC-0001”编号=“否”> <标题类型=“main”>背景</ title> >三种协议以便从<fi>固定漆粉扫描栏（EC 1.10.3.2）在壳聚糖上的Trametes Versicolor </ Fi>。特别地，壳聚糖用戊二醛和表氯醇官能化，以探索酶的缀合的程度，分别受羰基或环氧树脂改性表面的影响。此外，测试氧化程序以首次进行修饰，并首次改变酶的碳水化合物部分并利用其与壳聚糖胺基组连接。 </ p> </部分> <部分XML：ID =“JCTB5509-SEC-0002”编号=“否”> <标题类型=“main”>结果</ title> >漆件直接的系统与壳聚糖缀合似乎是最佳性能，因为它能够在30天的时间内保持100％的初始活性，并且在3催化循环后至少50％的起始活性。</ p> </段> <截面XML：ID =“JCTB5509-SEC-0003”编号=“否”> <标题类型=“main”>结论</ title> >方法显示出一种开发适合固定漆酶的生物催化剂的能力。所有这些都提供了不通过任何构象变化和活性口袋的任何变形来灭活酶的共价键。具体地，氧化方法证明是可以在不引入外部分子的情况下制备稳定化糖蛋白的可行性，表明漆酶的碳水化合物部分似乎与蛋白质的催化位点密切相关。还2017年化学工业协会</ p> </段> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-33908/'>《Journal of Chemical Technology & Biotechnology》</a> <b style="margin: 0 2px;">|</b><span>2018年第5期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Apriceno Azzurra&option=202" target="_blank" rel="nofollow">Apriceno Azzurra;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Girelli Anna Maria&option=202" target="_blank" rel="nofollow">Girelli Anna Maria;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Scuto Francesca Romana&option=202" target="_blank" rel="nofollow">Scuto Francesca Romana;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Department of ChemistrySapienza University of RomeRome Italy;</p> <p>Department of ChemistrySapienza University of RomeRome Italy;</p> <p>Department of ChemistrySapienza University of RomeRome Italy;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/20827.html" title="生物化学过程">生物化学过程;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Enzymes&option=203" rel="nofollow">Enzymes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Immobilisation&option=203" rel="nofollow">Immobilisation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Heterogeneous Catalysis&option=203" rel="nofollow">Heterogeneous Catalysis;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Catalyst Preparation&option=203" rel="nofollow">Catalyst Preparation;</a> </p> <div class="translation"> 机译：酶;固定化;异质催化;催化剂制剂; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704025422854.html">Design of a heterogeneous enzymatic catalyst on chitosan: investigation of the role of conjugation chemistry in the catalytic activity of a Laccase from <fi xmlns="http://www.wiley.com/namespaces/wiley">Trametes versicolor</fi>Trametes versicolor</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Apriceno Azzurra&option=202" target="_blank" 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Design of a heterogeneous enzymatic catalyst on chitosan: investigation of the role of conjugation chemistry in the catalytic activity of a Laccase from Trametes versicolorTrametes versicolor

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