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首页> 外文期刊>Journal of Chemical Technology & Biotechnology >Nitrogen removal by a metal-resistant bacterium, Pseudomonas putida ZN1, capable of heterotrophic nitrification-aerobic denitrification
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Nitrogen removal by a metal-resistant bacterium, Pseudomonas putida ZN1, capable of heterotrophic nitrification-aerobic denitrification

机译：通过耐金属细菌，假单胞菌普利达Zn1去除氮气，能够异养硝化的硝化 - 有氧脱氮

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摘要

BACKGROUND Bacteria capable of heterotrophic nitrification-aerobic denitrification have received increasing attention, and can achieve simultaneous nitrification and denitrification processes to accelerate nitrogen removal rate. However, heavy metals in industrial wastewater seriously affect the nitrogen removal by these bacteria. Thus, this work focused on the nitrogen removal ability of a metal-resistant bacterium Pseudomonas putida ZN1. RESULTS ZN1 effectively removed ammonium, nitrate and nitrite with maximum removal efficiencies of 97.47%, 86.08% and 71.57%, and it also preferred to utilize ammonium during the simultaneous nitrification and denitrification process. The inhibitory effect of heavy metals on ammonium removal decreased in the order Ni2+ > Cr6+ > Zn2+ > Cu2+, and copB-I was responsible for the higher copper tolerance of ZN1. The nitrogen removal pathway of ZN1 was proposed to be heterotrophic nitrification combined with aerobic denitrification through the nitrogen source utilization, gas detection, enzyme assay and gene amplification. CONCLUSION ZN1 exhibited excellent heterotrophic nitrification and aerobic denitrification capacity, and it effectively removed ammonium from wastewater containing the heavy metals Cu2+, Zn2+, Cr6+ and Ni2+. All results make strain ZN1 a promising candidate for future application in treating actual wastewater. (c) 2018 Society of Chemical Industry
机译：背景技术能够透过型硝化的硝化碳化反硝化的细菌受到越来越长，可以实现同时硝化和反硝化过程以加速氮去除率。然而，工业废水中的重金属严重影响这些细菌的氮气去除。因此，这项工作主要集中在金属抗性细菌假胶盖ZN1的氮去除能力上。结果Zn1有效地除去了铵，硝酸盐和亚硝酸盐，最大去除效率为97.47％，86.08％和71.57％，也优选在同时硝化和反硝化过程中使用铵。在Ni2 +> Cr6 +> Zn2 +> Cu2 + Zn2 +> Cu2 +的顺序中降低了重金属对铵的抑制作用，并且CopB-1负责Zn1的较高铜耐受性。 Zn1的氮去除途径被提出是通过氮源利用，气体检测，酶测定和基因扩增与需氧脱氮合并的异养硝化。结论Zn1表现出优异的异养硝化和有氧脱氮能力，有效地从含有重金属Cu2 +，Zn2 +，Cr6 +和Ni2 +的废水中除去铵。所有结果都使ZN1成为治疗实际废水的未来应用的有希望的候选者。 （c）2018化学工业协会

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