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首页> 外文期刊>Journal of Chemometrics >Method development for in situ study of marine vanadium peroxidase based on SERS and chemometrics
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Method development for in situ study of marine vanadium peroxidase based on SERS and chemometrics

机译：基于SERS和化学测量学的海洋钒过氧化物酶原位研究的方法

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摘要

Vanadium peroxidases from marine algae are responsible for the production of ozone-depleting compounds, volatile halogenated organic compounds (VHOC). Due to the impact the release of these compounds has on the global atmospheric and biogeochemical processes, there is an interest within marine sciences in developing analytical methods for studying the various aspects of the VHOC production, particularly in situ. This study aimed to provide new methods towards the development of in situ methods within marine sciences. We demonstrate the use of design of experiments together with orthogonal partial least squares (OPLS) and transposed orthogonal partial least squares (T-OPLS) to address the qualitative spectral analysis of an enzyme-buffer system. The measurements were performed with surface-enhanced Raman spectroscopy (SERS) on vanadium bromoperoxisase from the red algaeCorallina officinalis. The chemometric tools used aimed to provide greater insights into how factors such as time, amount of gold nanoparticles and enzyme concentration influence the spectral responses and whether there was any synergy between those factors. The results acquired in this report aim to support future method development of chemometrics within in situ applications in marine sciences.
机译：海藻藻类的钒过氧化物酶负责生产臭氧耗尽化合物，挥发性卤化有机化合物（VHOC）。由于影响这些化合物对全球大气和生物地球织化学过程的影响，在开发用于研究VHOC生产的各个方面的分析方法时，在海洋科学中存在兴趣，特别是原位。本研究旨在为在海洋科学中发展原位方法的发展。我们展示了使用实验的设计与正交的部分最小二乘（OPLS）和转置正交的部分最小二乘（T-OPLS）的使用，以解决酶缓冲系统的定性光谱分析。从Red AlgaeCorallina Officinalis用表面增强的拉曼光谱（SERS）用表面增强的拉曼光谱（SERS）进行测量。使用的化学工具旨在提供更大的洞察力，进入时间，金纳米颗粒的量，金纳米粒子和酶浓度的量如何影响光谱响应以及这些因素之间是否存在任何协同作用。在本报告中获得的结果旨在支持在海洋科学的原位应用中的化学计量学的未来方法开发。

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