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首页> 外文期刊>Journal of chemical neuroanatomy >Neurochemical fingerprinting of amygdalostriatal and intra-amygdaloid projections: a tracing–immunofluorescence study in the rat
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Neurochemical fingerprinting of amygdalostriatal and intra-amygdaloid projections: a tracing–immunofluorescence study in the rat

机译：Amygdalostriatal和杏仁内突出的神经化学指纹识别：大鼠追踪免疫荧光研究

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Amygdalostriatal and intra-amygdaloid fiber connectivity was studied in rats via injections of one of the tracers Phaseolus vulgaris leucoagglutinin (PHA-L) or biotinylated dextran amine (BDA) into various amygdaloid nuclei. To determine the neurotransmitter identity of labeled fibers we combined tracer detection with immunofluorescence staining, using antibodies against vesicular transporters (VTs) associated with glutamatergic (VGluT1, VGluT2) or GABAergic (VGAT) neurotransmission. High-magnification confocal laser scanning images were screened for overlap: occurrence inside tracer labeled fibers or axon terminals of immunofluorescence signal associated with one of the VTs.Labeled amygdalostriatal fibers were seen when tracer had been injected into the magnocellular and parvicellular portions of the basal amygdaloid nucleus and the lateral amygdaloid nucleus (nuclei belonging to 'cortical type' amygdaloid nuclei). Intra-amygdaloidal projection fibers were mostly found after tracer injections in the central and medial amygdaloid nuclei ('striatal type' amygdaloid nuclei).Terminals of tracer-labeled amygdalostriatal fibers contained immunofluorescence signal associated mostly with VGluT1 and to a lesser degree with VGluT2 or VGAT. Intra-amygdaloid labeled fibers showed colocalization mostly of VGluT1, followed by VGAT. VGluT2 co-occurred in a minority of intra-amygdaloid tracer-containing fiber terminals.We conclude from our observations that both amygdalostriatal and intra-amygdaloid projections, arising from, respectively, 'cortical type' and 'striatal type' amygdaloid nuclei contain strong glutamatergic and modest GABAergic components. The glutamatergic fibers express either VGluT1 or VGluT2. The absence in large numbers of tracer labeled fibers of expression of one of the selected VTs leads us to suspect that amygdalostriatal projection fibers may contain hitherto neglected neurotransmitters in these connections, e.g., aspartate.
机译：在大鼠中通过注射种子蛋白（Pha-L）或生物素化的葡聚糖胺（BDA）中的一种样品调导鼠或生物素化的葡聚糖胺（BDA）研究了杏仁孢子瘤纤维连通性。为了确定标记纤维的神经递质特性，我们将特拉克检测与免疫荧光染色组合，使用抗谷胱甘肽（Vglut1，Vglut2）或Gabaergic（Vgat）神经递血相关的囊泡转运蛋白（VTS）的抗体。筛选高倍率共聚焦激光扫描图像以重叠：当示踪剂注入基部杏仁醇的甲型和甲状腺细胞部分时，观察到与其中一个VTS.LABELED的氨基孢子纤维的标记纤维的出现标记的纤维或免疫荧光信号的轴突。核和横向杏仁核（属于'皮质型'氨基酸核的细胞核）。在中间杏仁核的示踪剂注射后，杏仁内突出纤维大多数发现（'纹状体型氨基酮核核）。特拉克标记的氨基孢子纤维纤维的终端含有大多数与VGLUT1和VGGAT的较小程度相关的免疫荧光信号。杏仁内标记的纤维大多是VGGAT的分致化。 Vglut2在少数含氨基喹喔啉纤维末端的少数群体中共发生。我们从我们的观察结果中得出结论，即Amygdalostriatoral和杏仁内突出的突起，分别引起的'皮质型'和'纹状体型'氨基喹啉核含有强谷氨酸酯和适度的加布力成分。谷氨酸纤维表达VGLUT1或VGLUT2。在大量的示踪剂标记的纤维中，其中一种选择的VTS的纤维引起我们怀疑氨基孢子瘤投影纤维可能含有迄今为止在这些连接中被忽略的神经递质，例如天冬氨酸。

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