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首页> 外文期刊>Journal of chemical neuroanatomy >Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides).
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Identification and characterization of a MBP isoform specific to hypothalamus in orange-spotted grouper (Epinephelus coioides).

机译：橙色斑点石斑鱼（Epinephelus Coioides）中丘脑中丘脑的MBP同种型的鉴定和表征。

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摘要

Myelin basic protein (MBP), as a major component of the myelin sheath, has been revealed to play an important role in forming and maintaining myelin structure in vertebrate nervous system. In teleost, hypothalamus is an instinctive brain center and plays significant roles in many physiological functions, such as energy metabolism, growth, reproduction, and stress response. In comparison with other MBP identified in vertebrates, a smallest MBP is cloned and identified from the orange-spotted grouper hypothalamic cDNA plasmid library in this study. RT-PCR analysis and Western blot detection indicate that the EcMBP is specific to hypothalamus, and expresses mainly in the tuberal hypothalamus in adult grouper. Immunofluorescence localization suggests that EcMBP should be expressed by oligodendrocytes, and the expressing cells should be concentrated in hypothalamus and the area surrounding hypothalamus, such as NPOpc, VC, DP, NLTm, and NDLI. The studies on EcMBP expression pattern and developmental behaviour in the brains of grouper embryos and larvae reveal that the EcMBP-expressing cells are only limited in a defined set of cells on the border of hypothalamus, and suggest that the EcMBP-expressing cells might be a subpopulation of oligodendrocyte progenitor cells. This study not only identifies a smallest MBP isoform specific to hypothalamus that can be used as a molecular marker of oligodendrocytes in fish, but also provides new insights for MBP evolution and cellular distribution.
机译：髓鞘基本蛋白（MBP）作为髓鞘的主要成分，已经揭示在脊椎动物神经系统中形成和维持髓鞘结构方面发挥着重要作用。在Textost，下丘脑是一种本能的脑中心，在许多生理功能中起着重要作用，例如能量代谢，生长，繁殖和压力反应。与脊椎动物中鉴定的其他MBP相比，在该研究中克隆并鉴定了最小的MBP并鉴定在该研究中的橙色斑点的石斑鱼下丘脑cDNA质粒。 RT-PCR分析和Western印迹检测表明ECMBP特异于下丘脑，主要表达在成人石斑鱼的结核下丘脑中。免疫荧光定位表明，ECMBP应该用少突胶质细胞表达，并且表达细胞应浓缩在下丘脑和周围丘脑区域的区域，例如NPOPC，VC，DP，NLTM和NDLI。 Grouper胚胎和幼虫大脑中ECMBP表达模式和发育行为的研究表明，ECMBP表达的细胞仅限于下丘脑边界的一组规定的细胞中，并表明ECMBP表达的细胞可能是一个寡核细胞祖细胞的亚迁移。这项研究不仅鉴定了特定于下丘脑的最小MBP同种型，可用作鱼中少突胶质细胞的分子标记，但也为MBP演化和细胞分布提供了新的见解。

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  • 来源
    《Journal of chemical neuroanatomy》 |2007年第2期|共13页
  • 作者

    Zhou L; Li CJ; Wang Y; Xia W; Yao B; Jin JY; Gui JF;

  • 作者单位

    State Key Laboratory of Freshwater Ecology and Biotechnology Wuhan Center for Developmental Biology Institute of Hydrobiology Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Wuhan China.;

    Department of Materials Science Kanagawa University Tsuchiya Hiratsuka Kanagawa 259-12 Japan;

    Department of Materials Science Kanagawa University Tsuchiya Hiratsuka Kanagawa 259-12 Japan;

    Department of Materials Science Kanagawa University Tsuchiya Hiratsuka Kanagawa 259-12 Japan;

    Department of Materials Science Kanagawa University Tsuchiya Hiratsuka Kanagawa 259-12 Japan;

    Department of Materials Science Kanagawa University Tsuchiya Hiratsuka Kanagawa 259-12 Japan;

    Department of Materials Science Kanagawa University Tsuchiya Hiratsuka Kanagawa 259-12 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 人体形态学;
  • 关键词

    Hypothalamus; Mean blood pressure; Myelin Sheath; Identification (Psychology); 下丘脑; 髓鞘; 认同(心理学);

    机译：Hypothalamus;Mean blood pressure;Myelin Sheath;Identification (Psychology);下丘脑;髓鞘;认同(心理学);

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