...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of chemical neuroanatomy >Phenotyping of sensory and sympathetic ganglion neurons of a galanin-overexpressing mouse--possible implications for pain processing.
【24h】

Phenotyping of sensory and sympathetic ganglion neurons of a galanin-overexpressing mouse--possible implications for pain processing.

机译：长丹林过表达小鼠的感觉和交感神经神经元的表型 - 疼痛加工的可能影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The distribution of galanin was studied in the lumbar 5 dorsal root ganglia (DRGs) and spinal cord, superior cervical ganglia (SCGs), and skin of transgenic mice overexpressing galanin under the dopamine beta-hydroxylase (DBH) promoter (GalOE-DBH mice) and in wild type (WT) mice. The DRGs and spinal cord were analysed before and after a unilateral, complete transection (axotomy) of the sciatic nerve and after dorsal rhizotomy. Both galanin protein and transcript were studied by, respectively, immunohistochemistry and in situ hybridization. Increased galanin expression was observed in several small, medium-sized and large DRG neuron profiles (NPs) in the naive transgenic mouse, frequently in neurons lacking calcitonin gene-related peptide (CGRP) and isolectin B4-binding. This lack of coexistence was particularly evident in the medium-sized/large NPs. In the dorsal horn of the spinal cord, no differences were detected between GalOE-DBH and WT mice, both displaying a strong galanin-positive neuropil in the superficial laminae of the dorsal horn, but the transgenic mice showed a more abundant galanin-positive innervation of the ventral horn. A 12-day dorsal rhizotomy, surprisingly, failed to alter the galanin staining patterns in the dorsal (and ventral) dorsal horn. Unilateral axotomy induced upregulation of galanin in DRG NPs of all sizes in both types of mouse. In the hindpaw skin, a profuse galanin-positive fiber plexus was observed in sweat glands and around blood vessels of the transgenic mice, being much more restricted in WT mice. Finally, GalOE mice exhibited a strong galanin-like immunoreactivity in most SCG NPs. The overexpression of the peptide in DRGs and SCGs was paralleled by increased mRNA levels. The present results show that overexpression of galanin under the control of the DBH promoter does not only occur, as expected in these mice, in noradrenline/adrenaline neurons but also in DRG neurons, particularly in large and medium-sized NPs. To what extent and how this overexpression pattern is related to the previously shown elevated pain threshold under normal and lesion conditions is discussed [Grass, S., Crawley, J.N., Xu, X.J., Wiesenfeld-Hallin, Z., 2003a. Reduced spinal cord sensitization to C-fibre stimulation in mice over-expressing galanin. Eur. J. Neurosci. 17, 1829-1832; Hygge-Blakeman, K., Brumovsky, P., Hao, J.X., Xu, X.J., Hokfelt, T., Crawley, J.N., Wiesenfeld-Hallin, Z., 2004. Galanin over-expression decreases the development of neuropathic pain-like behaviour in mice after partial sciatic nerve injury. Brain Res. 1025, 152-158].
机译：在腰部5背根神经节（DRGS）和脊髓，优质颈部神经节（SCG）以及过表达的甘油蛋白（Galoe-DBH小鼠）下的转基因小鼠皮肤的皮肤在野生型（WT）小鼠中。在坐骨神经和背根旋转术后单侧，完整的横衰期（腋窝术后，分析DRG和脊髓。通过分别通过免疫组织化学和原位杂交研究了甘丙蛋白蛋白和转录物。在幼稚转基因小鼠中的几种小，中大型和大型DRG神经元谱（NPS）中观察到增加的甘蛋白表达，经常在缺乏降钙素基因相关肽（CGRP）和Isolectin B4结合的神经元中。这种缺乏共存在中型/大型NPS中特别明显。在脊髓的背角中，Galoe-DBH和WT小鼠之间没有检测到差异，两者在背角的浅表椎间膜中显示强的野生蛋白阳性神经潜能，但转基因小鼠表现出更丰富的甘兰阳性内脏腹角。令人惊讶的是，12天的背离疏术，未能改变背部（和腹侧）背角中的环肽染色模式。单侧腋下诱导两种小鼠所有尺寸DRG NPS的甘丙蛋白的上调。在后爪皮肤中，在转基因小鼠的汗腺和血管周围观察到一种大量的野生植物阳性纤维丛，在WT小鼠中受到更大的限制。最后，Galoe小鼠在大多数SCG NPS中表现出具有强烈的甘嗪状免疫反应性。 DRGS和SCG中的肽的过表达通过增加的mRNA水平平行。本结果表明，在DBH启动子的控制下，在DBH启动子的控制下的过度表达不仅会在这些小鼠中预期的，而不是在诺拉克罗斯林/肾上腺素神经元中，但也在DRG神经元中，特别是在大中和中等大小的NPS中。在讨论了在正常和病变条件下的过表达模式与先前所示的升高疼痛阈值的程度和方式在多大程度上以及如何在正常和病变条件下进行讨论将脊髓敏化降低对表达甘油蛋白的小鼠C-纤维刺激。欧元。 J. Neurosci。 1829-1832; Hygge-Blakeman，K.，Brumovsky，P.，Hao，JX，Xu，XJ，Hokfelt，T.，Crawley，Jn，Wiesenfeld-Hallin，Z.，2004年。环肽过度表达降低了神经病疼痛的发展部分坐骨神经损伤后小鼠的行为。脑res。 1025,152-158]。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号