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首页> 外文期刊>Journal of chemical neuroanatomy >Embryonic intraventricular transplantation of neural stem cells augments inflammation-induced prenatal brain injury
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Embryonic intraventricular transplantation of neural stem cells augments inflammation-induced prenatal brain injury

机译：神经干细胞胚胎内移植增强炎症诱导的产前脑损伤

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ObjectivePrenatal brain injury results from undesirable circumstances during the embryonic development. Current endeavors for treating this complication are basically excluded to postnatal therapeutic approaches. Neural stem cell therapy has shown great promise for treating neurodevelopmental disorders. To our knowledge, this is the first study that investigates the therapeutic effect of in utero transplantation of neural stem cells (NSCs) in inflammation model of prenatal brain injury. MethodsTo induce prenatal injury, time-mated C57BL6J mice were intraperitoneally injected with 50?μg/kg lipopolysaccharide (LPS(on the day 15 of gestation. In the treatment group, NSCs were transplanted into the lateral ventricle of embryos on day 17 of gestation. The expression of GFAP, Iba-1, Olig2, and NeuN were assessed by real time PCR and immunohistochemistry. Changes in IL-6, TNF-α and IL-10 cytokines level, and caspase 3 activity were evaluated in the cortex of pups. ResultsIntrauterine transplanted NSCs homed to the brain cortex of offspring. Brain levels of pro-inflammatory cytokines showed a significant downward trend in the NSCs group. Furthermore, NSCs ameliorated inflammation-induced reactive microgliosis and astrogliosis as well as cellular degeneration. Apoptosis inhibition in the treated group was demonstrated by the decline in the caspase 3 activity and dark neurons. ConclusionThis study suggests a promising prospect to initiate the treatment of prenatal brain injury before birth by intrauterine transplantation of NSCs.
机译：目标培养脑损伤在胚胎发育期间的不良情况下产生。用于治疗这种并发症的目前的努力基本上被排除在产后治疗方法中。神经干细胞疗法对治疗神经发育障碍的良好承诺表现出很好。据我们所知，这是第一次研究神经干细胞（NSCs）在产前脑损伤炎症模型中的子宫内干细胞（NSC）的治疗效果。方法诱导产前损伤，腹膜腹腔内注射了50μg/ kg脂多糖（LPS（在妊娠的第15天）。在妊娠的第17天将NSCs移植到胚胎的侧脑室。通过实时PCR和免疫组织化学评估GFAP，IBA-1，OLIG2和NeUN的表达。在幼崽的皮质中评估IL-6，TNF-α和IL-10细胞因子水平的变化和Caspase 3活性。 CapuertRrautere移植到后代脑皮层的NSCs。促炎细胞因子的脑水平在NSCs组中显示出显着的下降趋势。此外，NSCs改善炎症诱导的活性微细胞源源晕和星球触减性，以及细胞变性。治疗中的凋亡抑制通过Caspase 3活性和黑暗神经元的下降证明了组。结论该研究表明，提出了一个有希望的前景，从而开始治疗产前脑损伤在出生前通过宫内移植NSCs。

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