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首页> 外文期刊>Journal of chemical neuroanatomy >Ethanolic extract from Hemidesmus indicus (Linn) displays otoprotectant activities on organotypic cultures without interfering on gentamicin uptake.
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Ethanolic extract from Hemidesmus indicus (Linn) displays otoprotectant activities on organotypic cultures without interfering on gentamicin uptake.

机译：来自Hemidemus Indicus（LINN）的乙醇提取物（LINN）显示有机型培养物的耳橡胶活性，而不会干扰庆大霉素摄取。

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摘要

The ethanolic extract from Hemidesmus indicus (Linn) (Apocynaceae) (Hie) was studied for its otoprotective effects in ex vivo rat organotypic model of gentamicin (GM) toxicity. In organ of Corti organotypic cultures (OC), GM can induce a fast dose-dependent apoptosis of hair cells (HC), both external and internal. We found that, after coadministration of GM and Hie to organotypic cultures, the extract was able to significantly counteract this toxic effect on HC, at the concentration of 25 and 50microg/ml. Interestingly, at these concentrations the extract was present in the cell medium at a concentration 1.6- and 3.3-fold lower than GM, suggesting its otoprotective activity could not merely due to an aspecific inhibition of GM entry. To support this hypothesis, we evaluated the amount of GM present in organotypic cultures after the coadministration of 1.5mg/ml GM and Hie, and found no significant reduction of GM uptake in the presence of 100microg/ml Hie. These data suggest the otoprotective action of Hie derives from specific inhibition of the apoptotic routine induced by GM treatment.
机译：研究了来自Hemidemus Indicus（LINOCIACEAE）（HIE）的乙醇萃取物（HIE）在庆大霉素（GM）毒性中的左体内大鼠有机型模型中的耳保护作用。在Corti有机型培养物的器官（OC）中，GM可以诱导外部和内部的毛细胞（HC）的快速剂量依赖性凋亡。我们发现，在GM和HIE与有机型培养的共同分析之后，提取物能够在25和50microg / mL的浓度下显着抵消对HC的这种毒性作用。有趣的是，在这些浓度下，提取物在细胞培养基中存在于比GM低1.6-和3.3倍的浓度为1.6-倍，表明其耳保护活性不能仅仅是由于GM进入的缺陷抑制。为了支持这一假设，我们评估了在1.5mg / ml GM和HIE的共同分析后有机型培养物中存在的血基数量，发现在100microg / ml HIE存在下没有显着降低GM吸收。这些数据表明HIE的耳保护作用导出来自GM治疗诱导的凋亡常规的特异性抑制。

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  • 来源
    《Journal of chemical neuroanatomy》 |2007年第4期|共6页
  • 作者

    Previati M; Corbacella E; Astolfi L; Catozzi M; Khan MT; Lampronti I; Gambari R; Capitani S; Martini A;

  • 作者单位

    Department of Morphology and Embryology Human Anatomy Division University of Ferrara via Fossato di Mortara 66 44100 Ferrara Italy. prm@unife.it;

    The Insitute for Solid Stte Physics The University of Tokyo 5-1-5Kashiwanoha Kashiwa-city Chiba 277 8581 Japan;

    The Insitute for Solid Stte Physics The University of Tokyo 5-1-5Kashiwanoha Kashiwa-city Chiba 277 8581 Japan;

    The Insitute for Solid Stte Physics The University of Tokyo 5-1-5Kashiwanoha Kashiwa-city Chiba 277 8581 Japan;

    The Insitute for Solid Stte Physics The University of Tokyo 5-1-5Kashiwanoha Kashiwa-city Chiba 277 8581 Japan;

    The Insitute for Solid Stte Physics The University of Tokyo 5-1-5Kashiwanoha Kashiwa-city Chiba 277 8581 Japan;

    The Insitute for Solid Stte Physics The University of Tokyo 5-1-5Kashiwanoha Kashiwa-city Chiba 277 8581 Japan;

    The Insitute for Solid Stte Physics The University of Tokyo 5-1-5Kashiwanoha Kashiwa-city Chiba 277 8581 Japan;

    The Insitute for Solid Stte Physics The University of Tokyo 5-1-5Kashiwanoha Kashiwa-city Chiba 277 8581 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 人体形态学;
  • 关键词

    Animals; Anti-Bacterial Agents; Cochlea; Dose-Response Relationship; Drug; 动物; 耳蜗; 剂量效应关系; 药物; 庆大霉素类; 神经保护药; 植物提取物; 大鼠;

    机译：Animals;Anti-Bacterial Agents;Cochlea;Dose-Response Relationship;Drug;动物;耳蜗;剂量效应关系;药物;庆大霉素类;神经保护药;植物提取物;大鼠;

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