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首页> 外文期刊>Journal of chemical neuroanatomy >Intrathecal transplantation of Wharton's jelly mesenchymal stem cells suppresses the NLRP1 inflammasome in the rat model of spinal cord injury
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Intrathecal transplantation of Wharton's jelly mesenchymal stem cells suppresses the NLRP1 inflammasome in the rat model of spinal cord injury

机译：沃顿果冻间充质干细胞的鞘内移植抑制了脊髓损伤大鼠模型中的NLRP1炎性

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摘要

After spinal cord injury (SCI) local inflammation is induced following secretion of interleukin-lbeta (IL-1 beta) and IL-18. It has been described that the secretion of IL-1 beta and IL-18 is mediated by a cytoplasmic multiprotein complex, termed inflammasome. Mesenchymal stem cells (MSCs) have been extensively used for treating inflammatory diseases in which they showed immunomodulation characteristics. We utilized the anti-inflammatory potential of Wharton's jelly mesenchymal stem cells (WJ-MSCs) to target inflammasome complex in rat SCI model. Real time-polymerase chain reaction, western blotting, and ELISA assay were done one week after SCI to measure the expression of the inflammasome components including NLRP1, ASC, and active caspase-1 as well as IL-1 beta, IL-18, and tumor necrosis factor-alpha (TNF-alpha). The histologic alteration and hind-limb locomotion were evaluated three weeks after injury by nissl staining and Basso, Beattie, Bresnahan (BBB), respectively. Our results showed that WJ-MSCs transplantation significantly decreased the SCI-induced expression of the evaluated factors in both mRNA and protein levels. In addition, WJ-MSCs significantly increased the number of normal-appearance neurons in the ventral horn of spinal cord. Noteworthy, these effects resulted in a significant improvement of motor function recovery. We conclude that inflammasome inhibition may be one of the mechanisms for the anti-inflammatory effect of MSCs in the SCI.
机译：脊髓损伤（SCI）局部炎症后诱导白细胞介素-1beta（IL-1β）和IL-18分泌。已经描述了IL-1β和IL-18的分泌是由细胞质多蛋白复合物介导的，称为炎症。间充质干细胞（MSCs）已广泛用于治疗它们显示出炎症性疾病的炎症性疾病。我们利用沃顿果冻间充质干细胞（WJ-MSC）的抗炎潜力来靶向大鼠SCI模型中的炎症组络合物。 SCI后一周进行实时聚合酶链反应，蛋白质印迹和ELISA测定，以测量包括NLRP1，ASC和活性CASPase-1的炎症组分的表达以及IL-1β，IL-18和肿瘤坏死因子-α（TNF-α）。在NISSL染色和Basso，Beattie，Bresnahan（BBB）伤害后三周评估组织学改变和后肢运动。我们的研究结果表明，WJ-MSCs移植显着降低了MRNA和蛋白质水平评估因子的SCI诱导的表达。此外，WJ-MSCs显着增加了脊髓腹侧喇叭中正面神经元的数量。值得注意的是，这些效果导致电机功能恢复的显着提高。我们得出结论，炎症组抑制可以是MSCs在SCI中的抗炎作用的机制之一。

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