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首页> 外文期刊>Journal of chemical neuroanatomy >Osmotic stress induces corticosteroid-binding globulin expression in the rat hypothalamo-hypophyseal system
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Osmotic stress induces corticosteroid-binding globulin expression in the rat hypothalamo-hypophyseal system

机译:渗透胁迫在大鼠下丘脑 - 衰弱系统中诱导皮质类固醇结合球蛋白表达

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摘要

Corticosteroid-binding globulin CBG is expressed in magnocellular hypothalamic nuclei, in part colocalized with vasopressin (VP) and oxytocin (OT). Here we subjected intact adult male rats to chronic osmotic stress to determine effects on distribution of CBG in VP and OT neurons and in neurons expressing corticotropin- releasing hormone (CRH). Drinking 2% NaCl solution for seven days resulted in increased CBG-immunoreactivity in magnocellular neurons. Triple immunofluorescence revealed increased colocalization with either VP, OT or CRH. Colocalization of CRH with VP was found only in a small portion of parvocellular neurons in the PVN. Most of the CBG-immunostained neurons within the magnocellular nuclei were devoid of CRH-immunoreactivity. Increased numbers of axons with colocalization of CBG and VP or OT were found in the internal zone of the median eminence (ME) of osmotically challenged rats. The external zone of the ME showed numerous CRH-positive neuronal projections. A small portion of them contained also CBG-immunofluorescence in both experimental animals and controls. Immunoassays of cerebrospinal fluid showed increased levels of CBG in osmotically stressed animals.
机译:皮质类固醇结合球蛋白CBG在甲型粒状下丘脑中表达,部分用血管加压素(vp)和催产素(OT)分致大致。在这里,我们对慢性渗透胁迫进行了完整的成年雄性大鼠,以确定对VP和OT神经元的CBG和表达CorticoTropin释放激素(CRH)的神经元的影响。饮用2%NaCl溶液七天导致甲型粒细胞神经元中的CBG免疫反应性增加。三重免疫荧光显示出与VP,OT或CRH的分致氢化增加。 CRH与VP的CROPalization仅在PVN中的一小部分细胞神经元中发现。大多数CBG免疫染色的玉米细胞核中的神经元缺乏CRH-免疫反应性。在渗透挑战性大鼠中位数(ME)的内部区域中发现了CBG和VP或OT的分层的轴突数增加。我的外部区域显示了许多CRH阳性神经元突起。它们的一小部分在实验动物和对照中也包含CBG免疫荧光。脑脊液的免疫测定显示出在渗透压的动物中增加的CBG水平。

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