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首页> 外文期刊>AIDS >Resumption of HIV replication is associated with monocyte/macrophage derived cytokine and chemokine changes: results from a large international clinical trial.
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Resumption of HIV replication is associated with monocyte/macrophage derived cytokine and chemokine changes: results from a large international clinical trial.

机译：HIV复制的恢复与单核细胞/巨噬细胞衍生的细胞因子和趋化因子的变化有关：一项大型国际临床试验的结果。

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BACKGROUND: There is increasing interest in the role of immune activation and inflammation in HIV disease, but data on direct effects of HIV replication on immune cell activation are limited. METHODS: High sensitivity multiplex bead array assays (MBAAs) were used to measure changes in plasma cytokines and chemokines [interleukin (IL)-1beta, IL-2, IL-6, IL-7, IL-8, IL-12p70, IL-17, tumor necrosis factor-alpha (TNFalpha), interferon-gamma, granulocyte macrophage colony-stimulating factor, IL-4, IL-5, IL-10, IL-13, CXCL10] from randomization (month 0) to month 2 in a random sample of 200 patients from both the drug conservation (DC) and viral suppression (VS) arms of the Strategies for Management of Antiretroviral Therapy (SMART) trial. IL-6 was also measured by ELISA. Data were evaluated using nonparametric correlation and censored parametric analysis of covariance and associations were declared as statistically significant when the Bonferroni-adjusted P-value was less than 0.003. RESULTS: Compared with the VS arm, significant increases were seen in the DC arm for TNFalpha (+0.34 log(e) pg/ml, P = 0.0001), IL-10 (+0.33 log(e) pg/ml, P = 0.00001) and CXCL10 (+0.66 log(e) pg/ml, P = 0.00001). IL-6 ELISA poorly correlated with IL-6 MBAA (Spearman's rho = 0.29, P = 0.0001). CONCLUSION: Resumption of HIV replication after ceasing antiretroviral therapy is associated predominantly with an increase of monocyte/macrophage-derived cytokines. Measurement of IL-6 levels may be affected by assay method and this should be considered in future studies of biomarkers.
机译：背景：人们对免疫激活和炎症在HIV疾病中的作用越来越感兴趣，但是有关HIV复制对免疫细胞激活的直接作用的数据有限。方法：使用高灵敏度的多重磁珠阵列测定法（MBAA）来测定血浆细胞因子和趋化因子的变化[白介素（IL）-1beta，IL-2，IL-6，IL-7，IL-8，IL-12p70，IL -17，肿瘤坏死因子-α（TNFalpha），干扰素-γ，粒细胞巨噬细胞集落刺激因子，IL-4，IL-5，IL-10，IL-13，CXCL10]从随机分组（第0个月）到第2个月从抗逆转录病毒疗法管理策略（SMART）试验的药物保护（DC）和病毒抑制（VS）部门随机抽取200名患者。还通过ELISA测量IL-6。使用非参数相关性评估数据，并检查协方差的删失参数分析，当Bonferroni调整的P值小于0.003时，关联被声明为具有统计学意义。结果：与VS组相比，DC组中TNFα（+0.34 log（e）pg / ml，P = 0.0001），IL-10（+0.33 log（e）pg / ml，P = 0.00001）和CXCL10（+0.66 log（e）pg / ml，P = 0.00001）。 IL-6 ELISA与IL-6 MBAA的相关性很弱（Spearman的rho = 0.29，P = 0.0001）。结论：停止抗逆转录病毒治疗后恢复HIV复制主要与单核细胞/巨噬细胞衍生的细胞因子增加有关。 IL-6水平的测定可能会受到测定方法的影响，在以后的生物标记研究中应考虑这一点。

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