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首页> 外文期刊>Journal of clinical laboratory analysis. >Overexpression miR‐211‐5p hinders the proliferation, migration, and invasion of thyroid tumor cells by downregulating SOX SOX 11
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Overexpression miR‐211‐5p hinders the proliferation, migration, and invasion of thyroid tumor cells by downregulating SOX SOX 11

机译：过度表达MIR-211-5P通过下调SOX SOX 11阻碍甲状腺肿瘤细胞的增殖，迁移和侵袭

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摘要

Purpose This study was aimed to investigate the relationship between miR‐211‐5p and SOX 11 , and the effects of their interaction on the proliferation, viability, and invasion of human thyroid cancer ( TC ) cells. Methods We used quantitative real‐time PCR ( qRT ‐ PCR ) to determine the expression of miR‐211‐5p and SOX 11 mRNA in the thyroid tumorous and the adjacent tissues. The target relationship between miR‐211‐5p and SOX 11 was confirmed using dual luciferase reporter gene assay. Flow cytometry, colony formation assay, Transwell assay, and MTT assay were performed to determine the cell‐cycle progression, cell apoptosis, proliferation and invasion, respectively. In addition, the tumor formation assay in nude mice was done to assess the effect of miR‐211‐5p on TC development in vivo. Results MiR‐211‐5p was underexpressed, whereas SOX 11 was overexpressed in TC . The overexpression of miR‐211‐5p inhibited the expression of SOX 11 . The cell cycle was arrested and the proliferation as well as invasiveness was suppressed by exogenous miR‐211‐5p in TC cell line. The antitumor role of miR‐211‐5p was proved by the animal experiment. Conclusion MiR‐211‐5p affected the viability, proliferation and invasion of TC by negatively regulating SOX 11 expression.
机译：目的本研究旨在探讨miR-211-5p和sox 11之间的关系，以及它们对人甲状腺癌（Tc）细胞的增殖，活力和侵袭的影响。方法使用定量实时PCR（QRT - PCR）来确定甲状腺肿瘤和相邻组织中miR-211-5p和Sox 11 mRNA的表达。使用双荧光素酶报告酶基因测定来确认miR-211-5p和Sox 11之间的靶关系。流式细胞术，菌落形成测定，Transwell测定和MTT测定分别测定细胞周期进展，细胞凋亡，增殖和侵袭。此外，裸鼠中的肿瘤形成测定是为了评估miR-211-5p对体内Tc发育的影响。结果miR-211-5p是缺陷的，而SOx 11在TC中过度表达。 miR-211-5p的过表达抑制SOX 11的表达。通过TC细胞系中的外源MiR-211-5P抑制细胞周期并抑制了增殖以及侵袭性。 MIR-211-5P的抗肿瘤作用是通过动物实验证明的。结论MIR-211-5P通过负调节SOX 11表达影响TC的活力，增殖和侵袭。

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  • 作者

    Wang Lei; Shen Yan‐feng; Shi Zhi‐min; Shang Xiao‐juan; Jin Dong‐ling; Xi Feng;

  • 作者单位

    Department of PathologyMedical College of Hebei University of EngineeringHandan Hebei Province China;

    Department of OncologyAffiliated Hospital of Hebei University of EngineeringHandan Hebei Province;

    Department of PathologyMedical College of Hebei University of EngineeringHandan Hebei Province China;

    Microscope RoomMedicine College of Hebei University of EngineeringHandan Hebei Province China;

    Department of PathologyMedical College of Hebei University of EngineeringHandan Hebei Province China;

    Department of PathologyMedical College of Hebei University of EngineeringHandan Hebei Province China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 诊断学;
  • 关键词

    in vivo experiments; miR‐211‐5p; SOX 11; thyroid cancer ( TC );

    机译：体内实验;miR-211-5p;sox 11;甲状腺癌（Tc）;

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