...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >An efficient procedure for preparing high-purity pingyangmycin and boanmycin from Streptomyces verticillus var. pingyangensis fermentation broth via macroporous cation-exchange resin and subsequent reversed-phase preparative chromatography
【24h】

An efficient procedure for preparing high-purity pingyangmycin and boanmycin from Streptomyces verticillus var. pingyangensis fermentation broth via macroporous cation-exchange resin and subsequent reversed-phase preparative chromatography

机译：从Streptomyces Verticillus Var中制备高纯度平阳霉素和Boanmycin的有效方法。 平阳肌发酵液通过大孔阳离子 - 交换树脂和随后的反相制备色谱法

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Pingyangmycin (PYM) and boanmycin (BAM), two individual components of bleomycin (bleomycin A5 and bleomycin A6), are glycopeptide antitumor antibiotics. An efficient procedure for the preparation of PYM and BAM from Streptomyces verticillus var. pingyangensis fermentation broth using macroporous cation-exchange (MCE) resin followed by medium-pressure preparative liquid chromatography (MPLC) based on monodisperse poly(styrene-co-divinylbenzene) (p(st-dvb)) microspheres was investigated in this paper. Nine frequently used MCE resins were screened by static adsorption and desorption to enrich PYM and BAM from the fermentation broth, and D157 resin was found to be the most effective. After one run of column-based dynamic adsorption and desorption, the contents of PYM and BAM were increased by factors of 13.8 and 12.1 with recovery yields of 84.21% and 81.47%, respectively. The enriched samples were subjected to MPLC with columns prepacked with the PolyRP 10-300 microspheres. The operational parameters of the MPLC, including the stationary phase and mobile phase compositions, sample/stationary phase ratio, sample loading scale and flow rate, were screened and optimized. The results showed that the separation and purification for PYM and BAM by MPLC were dramatically improved with a mobile phase modifier of 0.15 mol/L ammonium chloride aqueous solution, a flow rate of 10 mL/min and a sample/stationary phase ratio of 1.0:100 (m/v, g/mL), and PYM and BAM with purities of more than 98.65% and 99.12% were obtained, respectively. The total recoveries of PYM and BAM reached 75.38% and 70.31%. The separation and purification method is simple, efficient, energy-saving, environmentally friendly and suitable for the large-scale preparation of high-purity PYM and BAM from Streptomyces verticillus var. pingyangensis fermentation broth.
机译：平阳霉素（Pym）和Boanmycin（BAM），两种博来霉素（BLEOMYCIN A5和BLEOMYCIN A6）的单独组分，是糖肽抗肿瘤抗生素。从链霉菌霉菌var制备Pym和Bam的有效方法。采用大孔阳离子 - 交换（MCE）树脂的平壤发酵液，其次是基于单分散聚（苯乙烯 - 共二乙烯基苯）（P（ST-DVB））微球的中压制备液相色谱（MPLC）。通过静态吸附和解吸筛选九个经常使用的MCE树脂，以富集Pym和来自发酵液的Bam，并且发现D157树脂是最有效的。在一次基于柱的动态吸附和解吸之后，Pym和Bam的含量分别增加了13.8和12.1的因子，分别回收率为84.21％和81.47％。将富集的样品进行MPLC，用预先用PolyRP 10-300微球进行柱。筛选和优化了MPLC的操作参数，包括固定相和流动相组合物，样品/固定相比，样品加载量和流速。结果表明，用0.15mol / L氯化铵水溶液的流动相改性剂，10mL / min的流速和1.0的样品/固定相比，将MPLC和BAM的分离和纯化显着改善。获得100（m / v，g / ml）和pym和bam，纯度超过98.65％和99.12％。 Pym和Bam的总回收率达到75.38％和70.31％。分离和净化方法简单，高效，节能，环保，适用于高纯度Pym和Bam的大规模制备来自链霉菌Verticillus Var的大规模制备。平壤发酵肉汤。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号