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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Development of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for characterizing caffeine, methylliberine, and theacrine pharmacokinetics in humans
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Development of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for characterizing caffeine, methylliberine, and theacrine pharmacokinetics in humans

机译：液相色谱 - 串联质谱（LC-MS / MS）方法的开发，用于在人体中表征咖啡因，甲基丙氨酸和剧性药代动力学的特征

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摘要

Coffea liberica possesses stimulant properties without accumulating the methylxanthine caffeine. The basis for this peculiar observation is that methylurates (e.g., theacrine and methylliberine) have replaced caffeine. The stimulant properties of methylurates, alone and in combination with caffeine, have recently been investigated. However, human pharmacokinetics and LC-MS/MS methods for simultaneous measurement of methylxanthines and methylurates are lacking. To address this deficiency, we conducted a pharmacokinetic study in which subjects (n = 12) were orally administered caffeine (150 mg), methylliberine (Dynamine (TM), 100 mg), and theacrine (TeaCrine (R), 50 mg) followed by blood sampling over 24 h. Liquid-liquid extraction of plasma samples containing purine alkaloids and internal standard (C-13-Caffeine) were analyzed using a C18 reversed-phase column and gradient elution (acetonitrile and water, both containing 0.1% formic acid). A Waters Xevo TQ-S tandem mass spectrometer (positive mode) was used to detect caffeine, methylliberine, theacrine, and IS transitions of m/z 195.11 -> 138.01, 225.12 -> 168.02, 225.12 -> 167.95, and 198.1 -> 140.07, respectively. The method was validated for precision, accuracy, selectivity, and linearity and was successfully applied to characterize the oral pharmacokinetics of caffeine, methylliberine, and theacrine in human plasma. Successful development and application of LC-MS/MS-based methods such as ours for the simultaneous measurement of methylxanthines and methylurates are essential for the characterization of potential pharmacokinetic and pharmacodynamic interactions.
机译：Coffea Liberica具有刺激性的性质而不积累甲基黄嘌呤咖啡因。这种特殊观察的基础是甲基磺化酯（例如，临术和甲基丙氨酸）取代了咖啡因。最近研究了甲基磺化物，单独和与咖啡因组合的兴奋剂性质。然而，缺乏人体药代动力学和LC-MS / MS / MS方法，用于同时测量甲基黄嘌呤和甲磺酸盐。为了解决这种缺陷，我们进行了一种药代动力学研究，其中受试者（n = 12）是口服咖啡因（150mg），甲基丙氨酸（动态（Tm），100mg）和血液（茶叶（R），50mg）进行通过血液取样超过24小时。使用C18反相柱和梯度洗脱（含有0.1％甲酸的乙腈和水）分析含有嘌呤生物碱和内标（C-13-咖啡因）的液体 - 液 - 液萃取含有嘌呤生物碱和内标（C-13-咖啡因）的液体 - 液体萃取。 Waters Xevo TQ-S串联质谱仪（阳性模式）用于检测咖啡因，甲基丙氨酸，植物，以及M / Z 195.11-> 138.01,225.12 - > 168.02,225.12 - > 167.95和198.1 - > 140.07的转变， 分别。该方法被验证了精度，精度，选择性和线性度，并成功地应用于表征人血浆中咖啡因，甲基纤维和紫雷的口腔药代动力学。成功的开发和应用基于LC-MS / MS的方法，例如我们同时测量甲基黄嘌呤和甲基磺化物的方法对于潜在的药代动力学和药效学相互作用是必不可少的。

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