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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >High-throughput metabolomics screen coupled with multivariate statistical analysis identifies therapeutic targets in alcoholic liver disease rats using liquid chromatography-mass spectrometry
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High-throughput metabolomics screen coupled with multivariate statistical analysis identifies therapeutic targets in alcoholic liver disease rats using liquid chromatography-mass spectrometry

机译：高通量代谢组织筛选与多元统计学分析相结合，鉴定了使用液相色谱 - 质谱法的酒精性肝病大鼠治疗靶标

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摘要

Identification of metabolic alterations is useful for elucidating the pathophysiology and mass spectrometry-aided metabolomic, which provides small molecule metabolites to understand underlying mechanisms of complex diseases. In this work, we developed a novel liquid chromatography coupled with tandem mass spectrometry-based metabolomics screen coupled with multivariate statistical analysis workflow to obtaining the metabolite annotation, visualization and rapid characterization of small molecular metabolites, then revealed the altered metabolic pathway in a rat model of alcohol-induced liver damage rats as case study. The ions were detected in both positive and negative mode, and the key biomarkers were selected through multivariate statistical screening analysis. We employed the enhanced mass spectrometry coverage method to comprehensive analysis of the metabolite data. Importantly, we further find these small molecule metabolites were associated with multiple metabolic pathways. The elucidation of these altered metabolic pathways should help identify new and specific therapeutic targets. In addition, this metabolomics workflow coupled to LC-MS/MS has been successfully applied to the identification of small molecule metabolites in this complex disease. We strongly believe that metabolomics studies will benefit from incorporating liquid chromatography coupled with tandem mass spectrometry strategies.
机译：代谢改变的鉴定可用于阐明病理生理学和质谱辅助代谢物，其提供小分子代谢物，以了解复杂疾病的潜在机制。在这项工作中，我们开发了一种与多元统计分析工作流程相结合的新型液相色谱，与多变量统计分析工作流程相结合，以获得小分子代谢物的代谢物注释，可视化和快速表征，然后揭示了大鼠模型中改变的代谢途径酒精诱导的肝脏损伤大鼠作为案例研究。在阳性和阴性模式下检测离子，通过多元统计筛选分析选择关键的生物标志物。我们采用增强的质谱覆盖方法来综合分析代谢物数据。重要的是，我们进一步发现这些小分子代谢物与多种代谢途径有关。这些改变的代谢途径的阐明应该有助于识别新的和特异性治疗靶标。此外，已经成功地应用于LC-MS / MS的这种代谢组学工作流程以鉴定该复杂疾病中的小分子代谢物。我们强烈认为，代谢组科研究将纳入液相色谱掺入串联质谱策略。

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    Fang Heng; Zhang Ai-hua; Sun Hui; Yu Jing-bo; Wang Liang; Wang Xi-jun;

  • 作者单位

    Heilongjiang Univ Chinese Med Natl Chinmed Res Ctr Sino Amer Chinmed Technol Collaborat Ctr Natl TCM Key Lab Serum Pharmacochem Lab Metabol D Heping Rd 24 Harbin Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Natl Chinmed Res Ctr Sino Amer Chinmed Technol Collaborat Ctr Natl TCM Key Lab Serum Pharmacochem Lab Metabol D Heping Rd 24 Harbin Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Natl Chinmed Res Ctr Sino Amer Chinmed Technol Collaborat Ctr Natl TCM Key Lab Serum Pharmacochem Lab Metabol D Heping Rd 24 Harbin Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Natl Chinmed Res Ctr Sino Amer Chinmed Technol Collaborat Ctr Natl TCM Key Lab Serum Pharmacochem Lab Metabol D Heping Rd 24 Harbin Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Natl Chinmed Res Ctr Sino Amer Chinmed Technol Collaborat Ctr Natl TCM Key Lab Serum Pharmacochem Lab Metabol D Heping Rd 24 Harbin Heilongjiang Peoples R China;

    Heilongjiang Univ Chinese Med Natl Chinmed Res Ctr Sino Amer Chinmed Technol Collaborat Ctr Natl TCM Key Lab Serum Pharmacochem Lab Metabol D Heping Rd 24 Harbin Heilongjiang Peoples R China;

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  • 正文语种 eng
  • 中图分类 生物化学技术;
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