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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >Establishment of a rapid and sensitive UPLC-MS/MS method for pharmacokinetic determination of nine alkaloids of crude and processed Corydalis turtschaninovii Besser aqueous extracts in rat plasma
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Establishment of a rapid and sensitive UPLC-MS/MS method for pharmacokinetic determination of nine alkaloids of crude and processed Corydalis turtschaninovii Besser aqueous extracts in rat plasma

机译：建立一种快速敏感的UPLC-MS / MS / MS方法，用于药代动力学测定硫代喹啉的九核苷酸九种生物碱在大鼠等离子体中的二甲蛋白水提取物中

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摘要

Corydalis turtschaninovii (CT) is a traditional Chinese medicine which is known to have analgesic effects, and is under investigation for the management of chronic pain. Our study aims to establish a UPLC-MS/MS method for pharmacokinetic determination of nine bioactive alkaloids of raw and processed CT in rat plasma. Nitidine chloride was selected as internal standard. After protein precipitation with methanol, the plasma samples were separated on a reversed phased column with a mobile phase of acetonitrile and water (including 0.1% formic acid). The MRM parameters were optimized as follows: m/z 354.0 -> 188.1 for protopine, m/z 321.0 -> 293.1 for coptisine, m/z 371.1 -> 189.1 for allocryptopine, m/z 357.1 -> 193.3 for tetrahydropalmatine, m/z 324.1 -> 176.1 for tetrahydrocoptisine, m/z 340.1 -> 176.2 for tetrahydroberberine, m/z 368.1 -> 289.1 for corynoline, m/z 370.5 -> 192.1 for corydaline, m/z 367.2 -> 351.2 for dehydrocorydaline, and m/z 406.0 -> 300.3 for the IS. The linearity, accuracy, precision, stability, recovery and matrix effect of the method were well validated. This method was successfully employed for a pharmacokinetic study of raw and vinegar-processed CT in rats. The absorption of the nine alkaloids was accomplished in an hour. The double peak phenomenon of the nine alkaloids may be ascribed to enterohepatic recirculation. Compared with the raw group, AUC(0 -> t) and C(max )of the nine alkaloids were significantly elevated in the vinegar-processed group. Our findings suggest that vinegar-processing could increase the bioavailability of the nine alkaloids of CT in rats. The pharmacokinetic information obtained will provide basis for application of processed CT in future clinical therapy.
机译：Corydalis Turtschaninovii（CT）是一种众所周知的中药，其已知具有镇痛作用，并正在调查慢性疼痛的管理。我们的研究旨在建立对大鼠血浆中原料和加工CT的九种生物活性生物碱的药代动力学测定的UPLC-MS / MS方法。选择氯化萘氯化物作为内标。在用甲醇蛋白沉淀后，将等离子体样品在反相色谱柱上，具有乙腈和水的流动相和（包括0.1％甲酸）。 MRM参数如下优化：M / Z 354.0-> 188.1用于蛋白质，M / Z 321.0-> 293.1，用于Allocryptopine，M / Z 357.1-> 193.3用于四氢丙氨酸，M / Z 324.1 - > 176.1用于四氢钨单，M / Z 340.1 - > 176.2用于四氢铀蛋白，M / Z 368.1 - > 289.1用于植物碱，M / Z 370.5 - > 192.1，用于植物冬式，M / Z 367.2 - > 351.2用于脱氢性Daline，以及M / z 406.0 - > 300.3为此。该方法的线性，准确性，精度，稳定性，恢复和矩阵效应得到了良好的验证。该方法已成功用于大鼠原料和醋加工CT的药代动力学研究。在一小时内完成九种生物碱的吸收。九种生物碱的双峰现象可以归因于进肠呼吸缺水再循环。与原群，九生物碱的AUC（0-> T）和C（MAX）相比，醋加工组显着升高。我们的研究结果表明，醋 - 加工可以增加CT在大鼠中的九种生物碱的生物利用度。获得的药代动力学信息将为未来的临床疗法应用加工CT的应用。

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