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首页> 外文期刊>Journal of Cytology >Role of argyrophilic nucleolar organizer region staining in identification of malignant cells in effusion
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Role of argyrophilic nucleolar organizer region staining in identification of malignant cells in effusion

机译：艾滋病核仁组织区染色在积液中鉴定恶性细胞的作用

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摘要

Background: Cytological examination of effusions helps to differentiate between benign and malignant effusions, but fails a definitive diagnosis in a number of cases. The main problem here is to distinguish reactive mesothelial cells from neoplastic cells. Aim: In the present study, we used argyrophilic nucleolar organizer region (AgNOR) staining for diagnosis of benign and malignant effusions. Materials and Methods: In this study. 100 cases of effusion samples were taken and centrifuged. Four smearswere prepared from sediment, one each for Leishman, hematoxylin and eosin (H and E) staining and two for AgNOR staining. AgNORs were counted as black dots in the nuclei of all abnormal cells using ' 100 oil immersion objective. Their dispersion and shape was compared in benign, malignant and atypical cases. Results: Out of 50 pleural effusion samples. 29 were benign. 10 were malignant and 11 atypical. Among the 47 peritoneal effusion samples, 27 were benign. 16 were malignant and 4 atypical. Out of three cases of pericardial effusion samples, one was benign and two were malignant. All effusions were subjected to AgNOR staining. The benign group consisted of cells showing 1 to 2 dots of regular size and shape. In malignant group, 3 to 5 dots of variable size, shape and irregular contours were observed per cell. In atypical group, the reactive mesothelial cells showed 1 to 2 dots, malignant cells showed 3 to 4 irregular dots. Thus, a differentiation was noted between activated mesothelial cells and malignant cells, which was not possible in Leishman and H and E-stained smears alone. Conclusion: Therefore. AgNOR can be branded as an extremely useful additional diagnostic tool forcytodiagnosis of effusions.
机译：背景：患有的细胞学检查有助于区分良性和恶性的积液，但在许多情况下失败了明确的诊断。这里的主要问题是区分来自肿瘤细胞的反应性间皮细胞。目的：在本研究中，我们使用艾滋病核仁组织者区（Agnor）染色以诊断良性和恶性的积液。材料和方法：在本研究中。采集100例积液样品并离心。从沉淀物制备的四个苦味，一体为Leishman，血毒素和eosin（h和e）染色和两个用于术治疗。使用“100个油浸没物镜”的所有异常细胞的核数为黑点计算。它们的分散和形状在良性，恶性和非典型病例中进行了比较。结果：50个胸腔积液样品。 29是良性的。 10是恶性和11个非典型的。在47个腹膜积液样中，27个是良性的。 16是恶性和4个非典型的。三种心包积液样品中的三种情况下，一个是良性的，两种是恶性的。所有的积液都受到Agnor染色。良性组包括显示1至2个常规尺寸和形状的细胞。在恶性基团中，每个细胞观察到3至5点的可变尺寸，形状和不规则轮廓。在非典型组中，反应性间皮细胞显示1至2个点，恶性细胞显示3至4个不规则点。因此，在激活的间皮细胞和恶性细胞之间注意到了分化，在Leishman和H中是不可能的，并且单独的E染色涂抹。结论：因此。 Agnor可以作为一种极其有用的额外诊断工具，生育诊断为生效。

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  • 来源
    《Journal of Cytology》 |2011年第4期|共5页
  • 作者

    Meenu Gill; Uma Singh; Qury S Mahapatra; Sapna Gehlot; Veena Gupta; Rajeev Sen;

  • 作者单位

    Department of Pathology Pandit B.D. Sharma Post Graduate Institute of Medical Sciences Rohtak India;

    Department of Pathology Pandit B.D. Sharma Post Graduate Institute of Medical Sciences Rohtak India;

    Department of Pathology Pandit B.D. SharmaPost Graduate Institute of Medical Sciences Rohtak India;

    Department of Pathology Pandit B.D. Sharma Post Graduate Institute of Medical Sciences Rohtak India;

    Department of Pathology Pandit B.D. Sharma Post Graduate Institute of Medical Sciences Rohtak India;

    Department of Pathology Pandit B.D. Sharma Post Graduate Institute of Medical Sciences Rohtak India;

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  • 正文语种 eng
  • 中图分类 细胞生物学;
  • 关键词

    Argyrophilic nucleolar organizer region; effusion; mesothelial cells; neoplastic cells;

    机译：艾滋病核仁组织者区;积液;间皮细胞;肿瘤细胞;

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