...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Melanoma research >Use of multiple cytometric markers improves discrimination between benign and malignant melanocytic lesions: a study of DNA microdensitometry, karyometry, argyrophilic staining of nucleolar organizer regions and MIB1-Ki67 immunoreactivity.
【24h】

Use of multiple cytometric markers improves discrimination between benign and malignant melanocytic lesions: a study of DNA microdensitometry, karyometry, argyrophilic staining of nucleolar organizer regions and MIB1-Ki67 immunoreactivity.

机译：多种细胞标记物的使用改善了良性和恶性黑素细胞病变之间的区别：DNA微光密度法，karyometry，核仁组织区的嗜酸性染色和MIB1-Ki67免疫反应性的研究。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

SUMMARY: Confident separation of benign naevi and malignant melanoma can sometimes be very difficult using conventional microscopy. This study evaluated the combined diagnostic abilities of multiple cytometric markers in separating various types of naevi from melanomas. The lesions studied included 27 benign compound naevi, 20 dysplastic naevi, 10 Spitz naevi and 24 melanomas. The cytometric features investigated were: (i) nuclear DNA content and chromatin compactness, measured by video imaged DNA microdensitometry; (ii) nuclear morphology, measured by nuclear morphometry (karyometry); (iii) transcriptional activity of nucleolar organizer regions, measured as the number and size of argyrophilic staining of nucleolar organizer regions (AgNORs); and (iv) cellular proliferative activity detected by quantifying the immunoreactivity of MIB1-Ki67 antigen. These variables were evaluated in the superficial, middle and deep zones of each lesion. Using multivariate discriminant analysis, a total diagnostic effectiveness of 97% could be achieved in separating the benign and malignant melanocytic lesions by co-evaluating variables for DNA microdensitometry, karyometry and AgNORs. A diagnostic effectiveness of 100% could be achieved if further co-evaluation with MIB1-Ki67 immunoreactivity was performed. Our study suggests that co-evaluation of multiple cytometric markers can improve the diagnostic abilities of individual techniques in separating benign naevi from malignant melanomas. This may be of particular significance in the diagnosis of melanocytic lesions whose biological behaviour cannot be confidently predicted by their histological features using conventional microscopy.
机译：总结：使用传统的显微镜有时很难非常可靠地分离出良性痣和恶性黑色素瘤。这项研究评估了多种细胞标记物在区分各种类型的痣和黑色素瘤中的综合诊断能力。研究的病变包括27例良性复合物naevi，20例发育不良性naevi，10例Spitz naevi和24例黑色素瘤。研究的细胞计数特征是：（i）核DNA含量和染色质紧密度，通过视频成像的DNA显微光密度法测量; （ii）核形态学，用核形态学（karyometry）测量; （iii）核仁组织区的转录活性，以核仁组织区（AgNORs）嗜酸性染色的数量和大小来衡量; （iv）通过定量MIB1-Ki67抗原的免疫反应性检测的细胞增殖活性。在每个病变的浅表，中部和深部区域评估了这些变量。使用多变量判别分析，通过共同评估DNA微光密度测定法，karyometry和AgNORs变量，在分离良性和恶性黑素细胞病变方面可达到97％的总诊断效率。如果与MIB1-Ki67免疫反应性进行进一步共同评估，则可以实现100％的诊断效率。我们的研究表明，多种细胞标记物的共同评估可以提高将恶性黑色素瘤与良性痣分离的各种技术的诊断能力。这在诊断黑素细胞病变中可能具有特别重要的意义，这些黑素细胞病变的生物学行为无法使用常规显微镜通过组织学特征可靠地预测。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号