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首页> 外文期刊>Journal of diabetes research. >A Liquid-Based Cytology System, without the Use of Cytocentrifugation, for Detection of Podocytes in Urine Samples of Patients with Diabetic Nephropathy
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A Liquid-Based Cytology System, without the Use of Cytocentrifugation, for Detection of Podocytes in Urine Samples of Patients with Diabetic Nephropathy

机译：一种液体基细胞学系统，不使用细胞分离症，用于检测患有糖尿病肾病患者尿液样本中的哆哆醛

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Objective. Podocytes have highly differentiated functions and are extremely difficult to grow; thus, damage of podocytes is associated with glomerular dysfunction. Desquamated podocytes can be detected in urine of patients with severe renal impairment. Unlike the rapidly progressive glomerular damage in glomerulonephritis, only a few desquamated podocytes are usually detected in diabetic nephropathy (DN). It is not clear whether the low podocyte count in DN is due to limitation of the conventional method or true pathological feature. The aim of this study was to compare the conventional method with a newly modified method in detecting podocytes in morning urine samples of patients with DN. Materials and Methods. The study subjects were patients with type 2 diabetes. Urine samples from these patients were analyzed by the conventional method (Cytospin?) and the modified method (SurePath?). We determined the rate of detection of urinary podocytes and the number of detected cells. Results. The detection rate and podocyte count were significantly higher by the modified method than by the conventional method. The differences in the detection rates and numbers of podocytes were not significant between patients with normoalbuminuria and those with macroalbuminuria. However, they were significant in patients with microalbuminuria. The number of podocytes in the urine correlated significantly with the albumin-to-creatinine ratio, but not with the estimated glomerular filtration rate. Conclusions. The true number of urinary podocytes, as measured by the modified SurePath?-based method, in patients with DN is much higher than that estimated by the conventional method.
机译：客观的。大织物具有高度分化的功能，并且极难成长;因此，致孔的损伤与肾小球功能障碍有关。可以在严重肾损伤患者的尿液中检测脱皮诱饵。与肾小球肾炎中的迅速渐进的肾小球损伤不同，只有少数脱皮的诱惑肾病（DN）。目前尚不清楚DN中低泛骨细胞计数是否是由于传统方法或真实病理特征的限制。本研究的目的是将常规方法与新修饰的方法进行比较检测DN患者患者急性尿液样本中的哆哆醛。材料和方法。研究受试者是2型糖尿病患者。通过常规方法（Cytospin？）和修饰的方法分析来自这些患者的尿液样本，并改性方法（SERPATH？）。我们确定了尿饱和哆哆细胞的检测率和检测到的细胞的数量。结果。通过通过传统方法的改性方法，检测率和孔细胞计数显着更高。患有常规蛋白尿尿度和含有大核蛋氨酸的人的患者之间的检测率和多粒细胞数量的差异并不重要。然而，它们在微蛋白尿的患者中是显着的。尿液中的多粒细胞数与白蛋白 - 致肌酐比率显着相关，但不具有估计的肾小球过滤速率。结论。由DN的DN患者的修饰的SurePath的方法测量的尿箍细胞的真实数远高于传统方法估计的方法。

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