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首页> 外文期刊>Belgian Journal of Zoology >Expression of collapsin response mediator protein-4 (CRMP-4) in plastic brain areas of adult songbird brain
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Expression of collapsin response mediator protein-4 (CRMP-4) in plastic brain areas of adult songbird brain

机译：成年鸣禽脑的塑性脑区域中胶原蛋白反应介质蛋白4（CRMP-4）的表达

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摘要

Collapsin response mediator protein-4 (CRMP-4), a member of CRMP family, is involved in neuronal differentiation and regulation of synaptic rearrangement. Neurite growth and axonal reorganization still continue in the adult songbird brain. It is probable for CRMP-4 to play an important role during these processes. To address this issue, we first obtained CRMP-4 cDNA from the brain of songbird (Lonchura striata) by use of RT-PCR and 3' rapid amplification of cDNA ends (3'RACE). We then examined the expression of CRMP-4 mRNA and protein in some plastic brain regions, including the hippocampus, song control nuclei and the cerebellum. Double immunohistochemistry was employed to identify the phenotypes of CRMP-4 expressing cells. The results indicated that: 1) a cloned fragment of CRMP-4 cDNA (798bp) showed high similarity with those comparable cDNA of reported species, confirming the evolutionary conservation of CRMP-4 sequence; 2) both CRMP-4 mRNA and protein were found to be dominantly located in the hippocampus, song control nuclei and the cerebellum among the whole brain; 3) some CRMP-4 positive cells were also labeled by the antibody for HuD (immature neurons), but not for NeuN (mature neurons), and GFAP (astrocytes). The presence of CRMP-4 in the plastic regions suggests that CRMP-4 might be concerned with the neural plasticity of songbird brain.
机译：Collapsin反应介质蛋白4（CRMP-4）是CRMP家族的成员，参与神经元的分化和突触重排的调节。在成年鸣鸟脑中神经突的生长和轴突的重组仍在继续。 CRMP-4在这些过程中可能扮演重要角色。为了解决这个问题，我们首先使用RT-PCR和3'快速扩增cDNA末端（3'RACE）从鸣禽（Lonchura striata）的大脑中获得CRMP-4 cDNA。然后，我们检查了CRMP-4 mRNA和蛋白在一些可塑性大脑区域的表达，包括海马，歌曲控制核和小脑。采用双重免疫组织化学来鉴定表达CRMP-4的细胞的表型。结果表明：1）克隆的CRMP-4 cDNA片段（798bp）与报道种的可比cDNA具有高度相似性，证实了CRMP-4序列的进化保守性。 2）发现CRMP-4 mRNA和蛋白均位于整个大脑的海马，歌曲控制核和小脑中。 3）一些CRMP-4阳性细胞也被抗体标记为HuD（未成熟神经元），但未标记NeuN（成熟神经元）和GFAP（星形胶质细胞）。在塑性区域中CRMP-4的存在表明CRMP-4可能与鸣禽大脑的神经可塑性有关。

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