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首页> 外文期刊>Journal of dermatological science >The bioactivity of transforming growth factor-beta1 can be regulated via binding to dermal collagens in mink lung epithelial cells.
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The bioactivity of transforming growth factor-beta1 can be regulated via binding to dermal collagens in mink lung epithelial cells.

机译：转化生长因子-β1的生物活性可以通过与水貂肺上皮细胞中的皮肤胶原结合来调节。

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BACKGROUND: The bioactivity of transforming growth factor-beta1 (TGF-beta1) is known to be regulated by some components of the extracellular matrix (ECM), but the possibility that it might be regulated by collagen, the richest ECM component, has never been previously reported. OBJECTIVE: This study was designed to investigate the possible role that different types of collagens might play on the bioactivity of TGF-beta1. METHODS: The interaction of 125I-TGF-beta1 and various types of collagen was examined by a solid-phase assay and by a co-precipitation assay. The bioactivity of TGF-beta1 was assessed by a proliferation assay in which mink lung epithelial cells were examined in the presence and absence of collagens. RESULTS: Activated native dimeric TGF-beta1 bound to type I collagen in a dose-dependent manner, while monomeric TGF-beta1 bound poorly to the collagen. Type III collagen, and type I gelatin, a heat-denatured type I collagen, also showed a similar interaction with TGF-beta1, however, type IVcollagen showed a weak interaction. In the presence of types I and III collagens, the inhibitory effect of TGF-beta1 on the proliferation of mink lung epithelial cells was sustained, thus suggesting that the bioactivity of TGF-beta1 had been enhanced. Type I gelatin also enhanced the inhibition of cell growth, but its effect was weak in comparison with that of type I collagen. The amount of TGF-beta1 which remained intact in the conditioned medium after incubation with MLEC in the presence of types I and III collagens was more than that incubated without collagen. CONCLUSIONS: Our results suggest that types I and III collagens, the two most abundant components of the interstitial collagens, can potentially bind to activated TGF-beta1 and regulate the bioactivity of this growth factor, thereby possibly maintaining the biologically available TGF-beta1 level.
机译：背景：已知转化生长因子-β1（TGF-BETA1）的生物活性由细胞外基质（ECM）的一些组分调节，但它可能由胶原蛋白（最富裕的ECM组件）调节的可能性从未如此以前报道。目的：本研究旨在调查不同类型胶原蛋白可能在TGF-Beta1的生物活性发挥作用的可能作用。方法：通过固相测定和通过共沉淀测定检查125i-TGF-β1和各种类型的胶原蛋白的相互作用。通过增殖测定评估TGF-Beta1的生物活性，其中在存在和不存在胶原蛋白中检测水貂肺上皮细胞。结果：用剂量依赖性方式激活本地二聚体TGF-β1，其含量依赖于I型胶原蛋白，而单体TGF-β1与胶原蛋白相结合。 III型胶原蛋白和I型明胶，一种热变性I型胶原蛋白，也显示出与TGF-β1类似的相互作用，然而，IVcollagen型型相互作用。在I和III型胶原蛋白的存在下，TGF-BETA1对貂肺上皮细胞增殖的抑制作用持续，因此表明TGF-β1的生物活性已得到增强。 I型明胶还增强了对细胞生长的抑制，但与I型胶原蛋白相比，其效果较弱。在I和III型胶原蛋白的情况下与MLEC孵育后，在调节培养基中保持完整的TGF-β1的量大于没有胶原蛋白的孵育。结论：我们的研究结果表明，I和III型胶原蛋白，间质胶原蛋白的两个最丰富的组分可以潜在地与活化的TGF-β1结合，并调节该生长因子的生物活性，从而可能保持生物可用的TGF-β1水平。

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