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首页> 外文期刊>Biotechnology Progress >Improving the Fermentation Performance of Saccharomyces cerevisiae by Laccase During Ethanol Production from Steam-Exploded Wheat Straw at High-Substrate Loadings
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Improving the Fermentation Performance of Saccharomyces cerevisiae by Laccase During Ethanol Production from Steam-Exploded Wheat Straw at High-Substrate Loadings

机译：高底物负荷下蒸汽爆破麦秸生产乙醇过程中漆酶提高酿酒酵母的发酵性能

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摘要

Operating the saccharification and fermentation processes at high-substrate loadings is a key factor for making ethanol production from lignocellulosic biomass economically viable. However, increasing the substrate loading presents some disadvantages, including a higher concentration of inhibitors (furan derivatives, weak acids, and phenolic compounds) in the media, which negatively affect the fermentation performance. One strategy to eliminate soluble inhibitors is filtering and washing the pretreated material. In this study, it was observed that even if the material was previously washed, inhibitory compounds were released during the enzymatic hydrolysis step. Laccase enzymatic treatment was evaluated as a method to reduce these inhibitory effects. The laccase efficiency was analyzed in a presaccharification and simultaneous saccharification and fermentation process at high-substrate loadings. Water-insoluble solids fraction from steam-exploded wheat straw was used as substrate and Saccharomyces cerevisiae as fermenting microorganism. Laccase supplementation reduced strongly the phenolic content in the media, without affecting weak acids and fur an derivatives. This strategy resulted in an improved yeast performance during simultaneous saccharification and fermentation process, increasing significantly ethanol productivity.
机译：在高底物负载下操作糖化和发酵过程是使木质纤维素生物质生产乙醇经济可行的关键因素。然而，增加底物负载存在一些缺点，包括培养基中浓度较高的抑制剂（呋喃衍生物，弱酸和酚类化合物），这会对发酵性能产生负面影响。消除可溶性抑制剂的一种策略是过滤和洗涤预处理的物质。在该研究中，观察到即使预先洗涤了该材料，在酶促水解步骤中也会释放抑制性化合物。评价漆酶的酶处理作为减少这些抑制作用的方法。在预糖化，同时糖化和发酵过程中在高底物负载下分析了漆酶的效率。蒸汽炸麦秸的水不溶性固体部分用作底物，酿酒酵母用作发酵微生物。补充漆酶可大大降低培养基中的酚含量，而不会影响弱酸和衍生物。该策略在同时糖化和发酵过程中改善了酵母性能，从而显着提高了乙醇生产率。

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