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首页> 外文期刊>Biotechnology Progress >Influence of L-Homoarginine as an Analogue of L-Arginine on the Heat-Induced Aggregation of Proteins
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Influence of L-Homoarginine as an Analogue of L-Arginine on the Heat-Induced Aggregation of Proteins

机译：L-高精氨酸作为L-精氨酸类似物对蛋白质热诱导聚集的影响

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摘要

The influence of L-homoarginine on the heat-induced aggregation of three model proteins, i.e. porcine, mink, and human growth hormones was investigated by circular dichroism spectroscopy. It was found that the effect of L-homoarginine as an analogue of arginine depends on the concentration of the additive as well as the protein itself. L-Homoarginine increased the onset temperature of heat-induced aggregation of both porcine and mink growth hormones. However, the formation of human growth hormone aggregates was increased at low concentrations of L-homoarginine. Only at higher concentrations of the additive was the onset temperature of human growth hormone aggregation found to increase. Additional experiments of human growth hormone melting in the presence of histidine, lysine, and sodium chloride were performed. The effect of lysine was similar as in the presence of L-homoarginine. It follows that in protein formulations low concentrations of amino acids should be used with some precaution. At low concentration of additive, depending on the charge of both protein and amino acid used, the promotion of aggregation of unfolding intermediates may occur. (C) 2015 American Institute of Chemical Engineers
机译：通过圆二色谱法研究了L-高精氨酸对热诱导的三种模型蛋白，即猪，貂和人生长激素的聚集的影响。发现L-高精氨酸作为精氨酸类似物的作用取决于添加剂的浓度以及蛋白质本身。 L-高精氨酸提高了热诱导的猪和水貂生长激素聚集的起始温度。然而，在低浓度的L-高精氨酸下，人类生长激素聚集体的形成增加了。仅在较高浓度的添加剂下，人类生长激素聚集的起始温度才发现增加。在组氨酸，赖氨酸和氯化钠存在下进行人类生长激素融化的其他实验。赖氨酸的作用类似于存在L-高精氨酸的作用。因此，在蛋白质制剂中应谨慎使用低浓度的氨基酸。在低浓度添加剂下，取决于所用蛋白质和氨基酸的电荷，可能会促进未折叠中间体的聚集。 （C）2015美国化学工程师学会

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