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首页> 外文期刊>Belgian Journal of Zoology >A qualitative comparison of 3d visualization in xenopus laevis using a traditional method and a non-destructive method
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A qualitative comparison of 3d visualization in xenopus laevis using a traditional method and a non-destructive method

机译：使用传统方法和非破坏性方法对非洲爪蟾的3D可视化进行定性比较

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Many tools are currently available to investigate and visualize soft and hard tissues in animals both in high-resolution and three dimensions. The most popular and traditional method is based on destructive histological techniques. However, these techniques have some specific limitations. In order to avoid those limitations, various non-destructive approaches have surfaced in the last decades. One of those is micro-CT-scanning. In the best conditions, resolution achieved in micro-CT currently approaches that of standard histological protocols. In addition to bone, soft tissues can also be made visible through micro-CT-scanning. However, discriminating between structures of the same tissue and among different tissue types remains a challenge. An alternative approach, which has not yet been explored to its full potential for comparative anatomy studies, is Orthogonal-Plane Fluorescence Optical Sectioning (OPFOS) microscopy or tomography, also known as (Laser) Light Sheet based Fluorescence Microscopy (LSFM). In this study, we compare OPFOS with light microscopy, applying those techniques to the model organism Xenopus laevis. The potential of both methods for discrimination between different types of tissues, as well as different structures of the same tissue type, is tested and illustrated. Since the histological sections provided a better resolution, adjacent structures of the same tissue type could be discerned more easily compared to our OPFOS images. However, we obtained a more naturally-shaped 3D model of the musculoskeletal system of Xenopus laevis with OPFOS. An overview of the advantages and disadvantages of both techniques is given and their applicability for a wider scope of biological research is discussed.
机译：当前有许多工具可用于高分辨率和三维地研究和可视化动物的软组织和硬组织。最流行和传统的方法是基于破坏性的组织学技术。但是，这些技术有一些特定的限制。为了避免这些限制，在过去的几十年中出现了各种非破坏性方法。其中之一是微CT扫描。在最佳条件下，目前在微型CT中获得的分辨率接近标准组织学方案的分辨率。除了骨骼之外，还可以通过微型CT扫描使软组织可见。但是，区分相同组织的结构和不同组织类型的结构仍然是一个挑战。尚未被开发出用于比较解剖学研究的全部潜能的另一种方法是正交平面荧光光学切片（OPFOS）显微镜或层析成像，也称为（激光）光片荧光显微镜（LSFM）。在这项研究中，我们将OPFOS与光学显微镜进行了比较，并将这些技术应用于模型生物非洲爪蟾。测试和说明了两种区分不同类型的组织以及相同组织类型的不同结构的方法的潜力。由于组织学切片提供了更好的分辨率，因此与我们的OPFOS图像相比，可以更容易地识别出相同组织类型的相邻结构。但是，我们使用OPFOS获得了非洲爪蟾肌肉骨骼系统的更自然形状的3D模型。概述了这两种技术的优缺点，并讨论了它们在更广泛的生物学研究中的适用性。

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