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首页> 外文期刊>Journal of Economic Entomology >Optimizing Egg Recovery From Wild Northern Corn Rootworm Beetles (Coleoptera: Chrysomelidae)
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Optimizing Egg Recovery From Wild Northern Corn Rootworm Beetles (Coleoptera: Chrysomelidae)

机译:优化野生北玉米根虫甲虫的鸡蛋恢复(鞘翅目:Chrysomelidae)

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The northern corn rootworm, Diabrotica barberi Smith & Lawrence (Coleoptera: Chrysomelidae), is one of the most important insect pests in the U.S. Corn Belt. Efforts to obtain eggs from wild northern corn rootworm populations using techniques developed for other rootworm species have been unsuccessful due to lack of oviposition. In 2016, we evaluated four oviposition media in choice tests within each of three female densities in 30.5 x 30.5 x 30.5 cm BugDorm cages. The number of eggs laid per female was significantly affected by female density and the interaction of female density x oviposition media, but oviposition was relatively poor in all oviposition media (1.2 eggs per female when averaging the three female densities and all oviposition media). Single females were also evaluated in nonchoice assays in 6 cm x 6 cm x 8 cm clear plastic boxes and averaged up to 108 eggs per female depending on the oviposition media. In 2017, the cumulative number of eggs laid per female in boxes with one female was not significantly different from the number of eggs laid per female in boxes with 3 females. In 2018, the cumulative number of eggs laid per female was not significantly different between female densities of 1, 3, 5, or 10 females per box. Total egg production per box therefore increased as female density increased. More than 27,000 wild northern corn rootworm eggs were collected from just 190 females when collected relatively early in the field season. We now have an efficient and robust system for obtaining eggs from wild northern corn rootworm females.
机译:北玉米根虫,Diortboto Barberi Smith&Lawrence(Coleoptera:Chrysomelidae)是美国玉米皮带中最重要的害虫之一。由于缺乏产卵,使用为其他根茎种类的技术获得来自野性北部玉米根虫种群的鸡蛋的努力是不成功的。在2016年,我们在30.5 x 30.5 x 30.5 cm bugdorm笼中的三个女性密度中的每一个选择测试中评估了四个产卵介质。每个雌性的鸡蛋的数量受到雌性密度的显着影响,并且女性密度x产卵介质的相互作用,但在所有产卵介质中,产卵相对较差(当平均三个雌性密度和所有产卵介质时,每个女性1.2卵)。在6cm×6cm×8cm透明塑料盒中,还在非舍诺测定中评估单一的雌性,并且根据产卵介质,每个女性平均到每名108个鸡蛋。在2017年,每个女性在一个女性的女性中占鸡蛋的累积数量与每位女性的鸡蛋数量没有显着不同。 2018年,每名女性的累计鸡蛋数量在每箱1,3,5或10个女性的女性密度之间没有显着差异。随着女性密度增加,每个盒子的总鸡蛋产量增加。在野外季节相对早期收集的190名女性中,从190名女性收集了超过27,000个野生北玉米根卵。我们现在有一个高效且强大的系统,用于从野生北玉米根虫女性中获得鸡蛋。

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